Anti-VGLUT2 Antibody, clone 3C2, ZooMAb® Rabbit Monoclonal

Кат. номер

ZRB1170-25UL

ZRB1170-4X25UL

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.,
Specificity
Clone 3C2 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Vesicular glutamate transporter 2 (VGLUT2). It targets an epitope within 18 amino acids from the C-terminal region.
KLH-conjugated linear peptide corresponding to 18 amino acids from the C-terminal region of Rat Vesicular glutamate transporter 2 (VGLUT2).
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected VGLUT2 in rat cerebellum and mouse brain striatum tissue sections.
Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected VGLUT2 in mouse cerebellum and rat cerebral cortex tissue sections.
Note: Actual optimal working dilutions must be determined by the end user as specimens and experimental conditions may vary.
Anti-VGLUT2, clone 3C2 ZooMAb , Cat. No. ZRB1170, is a highly specific recombinant rabbit monoclonal antibody that detects Vesicular glutamate transporter 2 (VGLUT2) and has been tested for use in Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting.
Target description
Vesicular glutamate transporter 2 (UniProt Q9JI12; also known as Differentiation-associated BNPI, Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter, Solute carrier family 17 member 6, VGLUT2) is encoded by the Slc17a6 (also known as Dnpi, Vglut2) gene (Gene ID 84487) in rat species. VGLUT2 is a multi-pass membrane protein that mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neurons. Its N- and C-terminal tails (aa 1-71 and 499-582, respectively) are exposed at the cytoplasmic side. VGluT1- and VGluT2-expressing neurons represent the bulk of canonical glutamatergic neurons in the brain, with VGluT1 representing the major subtype and accounting for approximately 80% of total vesicular transport of glutamate in the brain. In contrast, VGluT3 is found in discrete population of non-glutamatergic neurons using transmitters other than glutamate, such as cholinergic interneurons in the dorsal and ventral striatum, subpopulations of GABAergic basket cells in the cortex and hippocampus, and serotoninergic neurons, where it is involved in more subtle modulation of local transmission. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose. Normal appearance is a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
300 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit (recombinant)
recombinant	expressed in HEK 293 cells
antibody form	purified antibody
antibody product type	primary antibodies
clone	3C2, recombinant monoclonal
product line	ZooMAb® learn more,
form	lyophilized
mol wt	calculated mol wt 64.58 kDa, observed mol wt ~60 kDa
species reactivity	rat, mouse
packaging	antibody small pack of 25 µL
greener alternative product characteristics	Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry,.
enhanced validation	recombinant expression Learn more about Antibody Enhanced Validation,
application(s)	immunofluorescence: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable (peptide)
isotype	IgG
conjugate	unconjugated
greener alternative category	Aligned,
shipped in	ambient
storage temp.	2-8°C

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable