Anti-Vimentin Antibody, clone 2I13 ZooMAb® Rabbit Monoclonal

Кат. номер

ZRB1584-4X25UL

ZRB1584-25UL

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.,
Specificity
Clone 2I13 is a rabbit recombinant monoclonal antibody that specifically detects Vimentin. It targets an epitope within 15 amino acids from the N-terminal half.
KLH-conjugated linear peptide corresponding to 15 amino acids from the N-terminal half of human Vimentin.
Anti-Vimentin, clone 2I13 ZooMAb, Cat. No. ZRB1584, is a recombinant Rabbit monoclonal antibody that specifically targets Vimentin and is tested for use in Affinity Binding Assay, Immunohistochemistry (Paraffin), and Western Blotting.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Vimentin in L6, U251, K562, and HEK293 cell lysates.
Affinity Binding Assay: A representative lot of this antibody bound Vimentin peptide with a KD of 5.5 x 10-7 in an affinity binding assay.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Vimentin in human kidney tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Target description
Vimentin (UniProt: P08670) is encoded by the VIM gene (Gene ID: 7431) in human. Vimentins are class-III intermediate filaments found in various non-muscle cells. It is the major subunit protein of the intermediate filaments of mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. It is usually expressed by cells undergoing cell-to-cell adhesion, EMT, and cytoskeleton remodeling. It is also involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin is also involved in positioning autophagosomes, lysosomes and the Golgi complex within the cell. It has also been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin consists of a central -helical rod domain. The N-terminal part of the rod domain is a coil 1 motif that is organized into sequences of seven amino acid residues, where every first and fourth residue is hydrophobic. The C-terminal half of the rod domain has a coil 2 motif that shows a hydrophobic pattern. The vimentin structure shown to be dynamic in cells and can be rearranged to form filaments or the soluble tetrameric protein. The head and tail domains of vimentin contain over 35 phosphorylation sites and phosphorylation of its serine residues is reported to inhibit its subunit polymerization, thus promoting the disassembly of vimentin filaments and increasing its solubility. These phosphorylations also regulate protein-protein interactions and intracellular signaling. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Danielsson, F., et al. (2018). Cells. 7(10); 147; Lowery, J., et al. (2015). J. Biol. Chem. 290(28); 17145-17153).
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	200,
recombinant	expressed in HEK 293 cells
antibody form	purified antibody
antibody product type	primary antibodies
clone	2I13, monoclonal, recombinant monoclonal
product line	ZooMAb® learn more,
form	lyophilized
mol wt	calculated mol wt 53.65 kDa, observed mol wt ~54 kDa
species reactivity	mouse, rat, human
species reactivity (predicted by homology)	bovine, porcine
packaging	antibody small pack of 25 µL
greener alternative product characteristics	Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry,.
enhanced validation	recombinant expression Learn more about Antibody Enhanced Validation,
application(s)	affinity binding assay: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable
isotype	IgG
conjugate	unconjugated
greener alternative category	Aligned,
shipped in	ambient
storage temp.	2-8°C

WGK Germany

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