Monoclonal Anti-MDMX antibody produced in mouse

MDL number:	MFCD00282354
NACRES:	NA.41

Кат. номер

M0445-100UL

M0445-200UL

Monoclonal Anti-MDMDX (mouse IgG1 isotype) is derived from the hybridoma MDMX-82 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice. MDMX belongs to the MDM family. The MDMX/ mouse double minute 4 (MDM4) gene encode a 490 amino acid protein. MDMX comprises a putative nuclear localization signal, a really interesting new gene (RING) finger domain in the C terminal and a p53-binding domain in the N terminal. MDMX has significant structural similarity to MDM2 E3 ubiquitin ligase.
Double minute 4 protein or MDMX is a nuclear protein. The gene encoding it is localized on human chromosome 1q32.1.
Specificity
Monoclonal Anti-MDMX recognizes human MDMX.
Monoclonal Anti-MDMX antibody is suitable for use in immunocytochemistry, immunoprecipitation, immunoblot (approx. 80 kDa), microarray, and ELISA. The product may also be used for western blot and for assessing ectopic expression of MDMX (using a conc. of 1-2 μg/mL in total cell extract of HEK 293T cells transfected with human MDMX).
Biochem/physiol Actions
MDMX is a structural homolog of MDM2 and inhibits the tumor suppressor, p53. MDMX also prevents MDM2-mediated degradation of p53, and subsequently regulates cellular levels of p53. MDMX also modulates cell growth and survival. Thus, MDMX can be useful as a therapeutic target in cancers, especially in tumors where native p53 functions can be restored . Monoclonal Anti-MDMX antibody is specific for human MDMX.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	200
biological source	mouse
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	MDMX-82, monoclonal
form	buffered aqueous solution
mol wt	antigen ~80 kDa
species reactivity	human
enhanced validation	recombinant expression
concentration	~2 mg/mL
application(s)	immunocytochemistry: suitable","immunoprecipitation (IP): suitable","microarray: suitable","western blot: 1-2 µg/mL using total cell extract of HEK 293T cells transfected with human MDMX
isotype	IgG1
conjugate	unconjugated
UniProt accession no.	O15151,
shipped in	dry ice
storage temp.	20°C
Gene Information	human ... MDM4(4194) mouse ... Mdm4(17248) rat ... Mdm4(304798)

Personal Protective Equipment	Eyeshields,, Gloves,, multi-purpose combination respirator cartridge (US),
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable