N-Glycosidase F, Elizabethkingia meningosepticum

CAS Number:	83534-39-8
Enzyme Commission (EC) Number:	3.5.1.52 ( BRENDA | IUBMB )
MDL number:	MFCD00131222

Кат. номер

362185-100UNU

362185-5000U

362185-100U

362185-100UCN

Native N-Glycosidase F from Elizabethkingia meningosepticum. Catalyzes the hydrolysis of asparagine-linked high mannose, as well as hybrid and complex oligosaccharides from glycoproteins. This enzyme will not cleave oligosaccharides containing 1-3-linked core fucose commonly found on plant glycoproteins. N-Glycosidase F will also fail to cleave if the asparagine to which the oligosaccharide is attached is not peptide-bonded to at least one amino acid residue at both the amino and carboxy termini.
Native N-Glycosidase F from Elizabethkingia meningosepticum. Catalyzes the hydrolysis of asparagine-linked high mannose, as well as hybrid and complex oligosaccharides from glycoproteins. This enzyme will not cleave oligosaccharides containing 1,3-linked core fucose commonly found on plant glycoproteins. N-Glycosidase F will also fail to cleave if the asparagine to which the oligosaccharide is attached is not peptide-bonded to at least one amino acid residue at both the amino and carboxy termini.
100 u in Plastic ampoule
Toxicity: Standard Handling (A)
One unit is defined as the amount of enzyme that will catalyze the release of N-linked oligosaccharides from 1.0 nmol denatured ribonuclease B per min at 37°C, pH 7.5. Cleavage is monitored by SDS-PAGE. One unit of N-Glycosidase F is equal to 1 IUB milliunit.
In 50 mM NaCl, 20 mM Tris-HCl, 1 mM EDTA, pH 7.5.
Means, R.E. and Desrosiers, R.C. 2000. J. Virol.74, 11181.
Fan, J.Q., and Lee, Y.C. 1997. J. Biol. Chem. 272, 27058.
Tarentino, A.L., and Plummer, T.H. 1994. Methods Enzymol. 230, 44.
Trimble, R.B., and Tarentino, A.L. 1991. J. Biol. Chem. 266, 1646.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

storage conditions	+2C to +8C
form	liquid
Quality Level	100
specific activity	20,000 units/mg protein, 4500 units/mL
Торговая марка	Calbiochem®
storage condition	do not freeze
foreign activity	Proteases, none detected
shipped in	wet ice
storage temp.	2-8°C

WGK Germany	WGK 2
Flash Point F	Not applicable
Flash Point C	Not applicable