Ascentis® Express 90 Biphenyl, 5.0 m HPLC Column 584592-U

Ascentis® Express 90 Biphenyl is a high-speed, high-performance liquid chromatography column based on a superficially porous Fused-Core® particle design. The particle provides a thin porous shell of high-purity silica surrounding a solid silica core. This particle design exhibits very high column efficiency due to the shallow diffusion paths in the 0.6 µm thick porous shell and the small overall particle size of 5 µm. The bonded, densely endcapped, dimethyl-biphenyl stationary phase provides a stable, reversed phase packing with enhanced pi-pi and mild steric interactions due to the two sequential phenyl groups bonded to the base silica. The 90 Biphenyl can be used for basic, acidic, or neutral compounds with alternate selectivity from C18 and is especially useful for separating peaks that elute early on C18 and other phenyl phases.
Analysis Note
Target analytes include electron-poor molecules, aromatic or unsaturated compounds (ketones, nitriles, alkenes).
Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.

material	stainless steel column
Agency/Method	suitable for USP L11
product line	Ascentis®
feature	endcapped
packaging	1 ea of
extent of labeling	5.5% Carbon loading
application(s)	HPLC: suitable, LC/MS: suitable
column L x I.D.	15 cm x 3 mm
matrix	Fused-Core® particle platform, superficially porous particle
matrix active group	Biphenyl (dimethylbiphenyl) bonding phase
particle size	5.0 µm
pore size	90 A pore size
surface area	90 m2/g
operating pH range	2 - 9
separation technique	reversed phase