LA-N-2

Кат. номер

6041202-DNA-5UG

6041202-CDNA-20UL

6041202-1VL

6041202-RNA-5UG

Human Neuroblastoma
Established by Seeger et al., (1977) from the primary site of a noncatecholamine-producing neuroblastoma of a 3-year-old female with clinical Stage IV neuroblastoma. The culture consists mostly of large, elongated cells with processes with some small round cells adhering to the elongated cells. The cells are tumourigenic in nude mice.
EMEM (with non-essential amino acids) and Ham′s F12 (1:1 mixture) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)
For routine maintenance, split cultures after they have become dense i.e. once every 3-4 weeks at a 1:20 to 1:50 ratio; 8% CO2; 37oC. The culture consists of large elongated cells with processes and small round cells. The latter tend to grow in dense clusters on the former and detach readily. Most cells are moderately adherent. Remove attached cells from the substrate with trypsin/EDTA. Cells will detach in 5-10 minutes. When resuscitated from a frozen ampoule cells may appear dead after a day but reattach and resume growth within 2-3 days. Cells grow best and are most adherent on a plastic substrate in medium at a pH of 6.9 - 7.2; they do not tolerate more alkaline pH well. Cultures hold well at high density with periodic medium changes.
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biological source	human nerve
growth mode	Not specified
karyotype	Modal no. 73, range 46 - 73
morphology	Large elongated cells + small round cells in clusters
products	Not specified
receptors	Not specified
application(s)	cell culture | mammalian: suitable
relevant disease(s)	cancer
shipped in	dry ice
storage temp.	−196°C

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NONH for all modes of transport