Y1

Кат. номер

85062807-DNA-5UG

85062807-RNA-5UG

85062807-1VL

Mouse LAF1 adrenal cortex tumour
This cell line is a cloned strain from an adrenal cortex tumour cell line of a male LAF1 mouse. This strain was isolated after alternate passage in tumours in vivo and in cultures in vitro . The isolated clones are reported by the original depositor to produce steroid hormones at a high rate (4ug/mg/hr) under ACTH stimulation and cells round up within minutes of ACTH treatment. The hormone produced is reported to be ketosteroid with a hydroxy group at the carbon 20 rather than the usual ketosteroid configuration. However, a laboratory independent to the depositor has since (Dec 2000) tested this steroid producing property using the same method and after 1 hour of stimulation could report no rounding up or change in morphology and a very low cAMP measurement. This laboratory did find that the cells round up when exposed to forskolin (acting through adenylate cyclase) and the original depositor reported morphological changes in response to cholera toxin.
Cloning and hormone studies
Ham′s F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
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biological source	mouse adrenal gland (cortex)
growth mode	Adherent
karyotype	2n = 40
morphology	Epithelial
products	Steroid hormone
receptors	Not specified
application(s)	cell culture | mammalian: suitable
relevant disease(s)	cancer
shipped in	dry ice
storage temp.	−196°C

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