Aconitase Activity Assay Kit MAK051-1KT

NACRES:

NA.84

The aconitases are a family of iron-sulfur containing enzymes that catalyzes the isomerization of citrate to isocitrate. Two aconitases, encoded by different genes, have been identified in eukaryotes. Aconitase 1 (ACO1, c-aconitase) is a cytosolic enzyme while Aconitase 2 (ACO2, m-aconitase) is a mitochondrial enzyme that functions in the tricarboxylic acid cycle. Aconitases are reversibly inactivated by oxidative stress, and aconitase activity in cells and tissues has been used as a biomarker for oxidative damage.
Aconitase Activity Assay Kit has been used to measure the activity of cytosolic and mitochondrial aconitase enzyme.
Compatible with high-throughput handling systems.
Suitable for the measurement of aconitase activity in biological samples including tissue and cells
The Aconitase Activity Assay kit provides a simple and direct procedure for measuring Aconitase activity in a variety of samples. Aconitase activity is determined in a coupled enzyme reaction in which citrate is converted to isocitrate by aconitase. This results in a colorimetric (450 nm) product proportional to the enzymatic activity present. One unit of aconitase is the amount of enzyme that will isomerize 1.0 μmole of citrate from isocitrate per minute at pH 7.4 at 25 °C.

usage	sufficient for 100 colorimetric tests
detection method	colorimetric
storage temp.	2-8°C
Gene Information	human ... ACO1(48), ACO2(50) mouse ... ACO1(11428), ACO2(11429) rat ... ACO1(50655), ACO2(79250)

pictograms	GHS05,GHS08
signalword	Danger
hcodes	H314 - H334
pcodes	P261 - P280 - P284 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
RIDADR	NONH for all modes of transport