Alpha Galactosidase (-Gal) Activity Assay Kit (Fluorometric) MAK390-1KT

Alpha-Galactosidase (α-Gal) hydrolyzes alpha-galactosyl moieties found in glycolipids and glycoproteins. In mammals, a-Gal hydrolyzes poly- and oligosaccharides commonly found in dietary sources that are difficult to digest. Therefore, α-Gal is used in dietary supplements that help to reduce the production of intestinal gases due to consumption of certain foods. It is known total α-Gal activity is due to two major isozymes with unique, yet different thermostability profiles. Alpha-Galactosidase A, is thermolabile and represents approximately 90% of total α-Gal activity. Fabry Disease, a lysosomal disease disorder, is characterized by mutations in alpha-Galactosidase A. These mutations cause abnormal accumulation of glycosphingolipids in lysosomes.
The Alpha Galactosidase (-Gal) Activity Assay Kit:

• Detection limit: < 0.1µU.
• Specific: does not detect beta galactosidase activity.

The kit is suitable for the detection of α-Galactosidase activity in tissue homogenates (kidney, etc.), cell lysates (U937, etc.) and biological fluids (saliva, etc.).
The Alpha Galactosidase (-Gal) Activity Assay Kit provides a simple, rapid way to monitor total -Gal activity in a wide variety of biological samples. In this kit, -Gal cleaves a synthetic specific substrate releasing a fluorophore, which can be easily quantified (ex = 360 nm/em = 445 nm). The assay is specific (beta galactosidase activity is not detected), sensitive and can detect as low as 0.1 mU of -Galactosidase activity.

Quality Level	100,
detection method	fluorometric
storage temp.	−20°C