Human Calpain 1 Inhibitor Screening Kit MAK210-1KT

NACRES:

NA.25

Mammalian calpains are calcium-activated, neutral, non-lysosomal, intracellular proteases that may be ubiquitous, stomach-specific, or muscle-specific. Calpain 1 is a ubiquitous protease that is involved in calcium homeostasis and the regulation of apoptosis. In cardiomyocytes, activated calpain 1 induces apoptosis by BID cleavage and release of cytochrome c from the mitochondria.
The Human Calpain 1 Inhibitor Screening Kit is a rapid, simple and sensitive assay that is suitable for high-throughput screening of Calpain 1 inhibitors. Calpain 1 activity is measured by cleaving a synthetic 7-amino-4-trifluoromethylcoumarin (AFC)-based substrate (Ac-LLY-AFC) to yield AFC, a fluorescent product (ex = 400/em = 505 nm), proportional to the enzymatic activity present.
Compatible with high-throughput handling systems.
Suitable for the screening of inhibitors of human Calpain 1.
The Human Calpain 1 Inhibitor Screening Kit is a rapid, simple and sensitive assay that is suitable for high-throughput screening of Calpain 1 inhibitors. Calpain 1 activity is measured by cleaving a synthetic 7-amino-4-trifluoromethylcoumarin (AFC)-based substrate (Ac-LLY-AFC) to yield AFC, a fluorescent product (ex = 400/em = 505 nm), proportional to the enzymatic activity present.

usage	sufficient for 100 fluorometric tests
detection method	fluorometric
shipped in	dry ice
storage temp.	−70°C
Gene Information	human ... CAPN1(823)

RIDADR	UN 3316 9
WGK Germany	WGK 3
Flash Point F	188.6 °F - closed cup
Flash Point C	87 °C - closed cup