Mannosidase Assay Kit MAK318-1KT

NACRES:

NA.84

α-Mannosidase (AMA) is an enzyme which catalyzes the cleavage of the alpha form of mannose. α-Mannosidase assists in the breakdown of complex sugars from glycoproteins in the lysosome. Defective AMA or deficient AMA activity causes α-mannosidosis and leads to deterioration of the central nervous system in children.
Compatible with high-throughput handling systems.
Suitable for determining α-Mannosidase (AMA) activity in a variety of biological samples such as plasma, serum, tissue, and cell culture media.
The -Mannosidase Activity Assay Kit provides a simple and high-throughput adaptable assay for determining -Mannosidase activity in a variety of biological samples such as plasma, serum, tissue, and cell culture media. In this assay 4-nitrophenol is cleaved from a synthetic substrate. Nitrophenol becomes intensely colored with the addition of the stop reagent, producing a colorimetric (405 nm) result, directly proportional to the enzyme activity in the sample.
One unit of AMA is the amount of enzyme that will convert 1.0 µmole of 4-Nitrophenyl--D-mannopyranoside to 4-Nitrophenol and -D-Mannose per minute at 25 °C and pH 4.5. The kit has a linear detection range of 1–250 unit/L for a 10 minute reaction in a 96 well format.

detection method	colorimetric
storage temp.	2-8°C

pictograms	GHS07
signalword	Warning
hcodes	H319
pcodes	P264 - P280 - P305 + P351 + P338 - P337 + P313
RIDADR	NONH for all modes of transport
Flash Point F	Not applicable
Flash Point C	Not applicable