Lipopolysaccharides from Proteus mirabilis

EC Number:

297-473-0

Кат. номер

SMB00704-5MG

SMB00704-5MG-PW

Lipopolysaccharides (LPS′s) are are complex glycolipids that are characteristic components of the cell wall of Gram-negative bacteria. They consist of a lipid A moiety linked to an antigenic O-polysaccharide.
Biochem/physiol Actions
Lipopolysaccharide (LPS) and its lipid A moiety stimulate cells of the innate immune system by the toll-like receptor 4 (TLR4), a member of the toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs). Lipid A plays a role in the biological properties attributed to endotoxic LPS. LPS is a highly immunogenic antigen which has the ability to enhance immune responses.
Proteus mirabilis is a Gram-negative facultative anaerobic rod-shaped bacterium. Bacteria of the genus Proteus of the family Enterobacteriaceae are opportunistic human pathogens responsible for wound and burn infections as well as skin, eye, ear, nose, throat, urinary tract, and gastrointestinal infections and bacteremias. The most common infection involving P. mirabilis occurs when the bacteria, which is a member of the natural intestinal flora, moves to the urethra and urinary bladder causing urinary tract infection.
The outer-membrane lipopolysaccharide is considered an important virulence factor of Proteus. It has been suggested that the immunological response against P.mirabilis LPS might play a role in rheumatoid arthritis. A possible correlation between the abundance of P.mirabilis in the intestine and obesity was suggested recently. The purified LPS from P. mirabilis OXK is used in the Weil-Felix test for the diagnosis of rickettsiosis (scrub typhus) caused by the bacterium Orientia (Rickettsia) tsutsugamushi.
Preparation Note
Soluble in water and cell culture medium DMEM (1 mg/mL) yielding a clear to slightly hazy solution.

Quality Level	200,
biological source	bacterial (Proteus mirabilis)
form	lyophilized powder
purified by	phenol extraction
shipped in	ambient
storage temp.	2-8°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable