Mutanolysin from Streptomyces globisporus ATCC 21553

CAS Number:	55466-22-3
MDL number:	MFCD00131635

Кат. номер

SAE0092-10KU

SAE0092-10KU-PW

Provides gentle cell lysis for the isolation of easily degradable biomolecules and RNA from bacteria. It has been used in the formation of spheroplasts for isolation of DNA.
Purified Mutanolysin SAE0092 undergoes strict quality control testing to ensure the absence of detectable levels of contaminating DNA using 35 cycles PCR amplification of 16S and 18S rDNA using universal primer sets.
Mutanolysin is a muralytic enzyme, suitable for usage in isolation of nucleic acids, from Gram-positive bacteria (e.g. Listeria, Lactobacillus, Lactococcus),(8) and also from other bacteria that are difficult to lyse with lysozyme only.
Biochem/physiol Actions
Mutanolysin is an N-acetylmuramidase. Like lysozyme, it is a muralytic enzyme that cleaves the -N-acetylmuramyl-(14)-N-acetylglucosamine linkage of the bacterial cell wall polymer peptidoglycan-polysaccharide. Its carboxy terminal moieties are involved in the recognition and binding of unique cell wall polymers. Mutanolysin lyses Listeria and other Gram-positive bacteria such as Lactobacillus and Lactococcus.
Unit Definition
One unit will produce a ΔA600 of 0.01 per minute at pH 6.0 at 37 °C in a 1 mL volume using a suspension of Streptococcus faecalis cell wall as substrate.
Physical form
Lyophilized powder containing Ficoll® and sodium succinate buffer salts
Ficoll is a registered trademark of Cytiva

form	lyophilized powder
specific activity	4000 units/mg protein (biuret)
mol wt	23 kDa, ~18.5 kDa
shipped in	wet ice
storage temp.	20°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable