Thermolysin from Geobacillus stearothermophilus

CAS Number:	9073-78-3
Enzyme Commission (EC) Number:	3.4.24.27 ( BRENDA | IUBMB )
EC Number:	232-973-4
MDL number:	MFCD00165549
eCl@ss:	32160410

Кат. номер

P1512-1G

P1512-100MG

P1512-250MG

P1512-25MG

P1512-10MG

Thermolysin is a protease that has specificity different from other proteases available for sequence investigations.
Thermolysin has been shown to have a prosequeence that acts as an intramolecular chaperone in vivo. It has also been used in a study to investigate the effects of sodium chloride on thermal stability and catalytic activity.
Thermolysin is also commonly used for the commercial synthesis of N-(benzyloxycarbonyl)-L-aspartyl-L-phenylalanine methyl ester, the precursor for the artificial sweetener aspartame.
Non-specific Protease Activity Assay Video
A thermostable (thermophilic) extracellular metalloendopeptidase containing four calcium ions. Cofactors are zinc and calcium. Hydrolyzes protein bonds on the N-terminal side of hydrophobic amino acid residues. The pH optimum is 8.0 and the optimal temperature for activity is 70 °C. Considerably stable from pH 5 to 9.5. Thermolysin has a low cleavage specificity, therefore, it produces a number of short fragments that are suitable for sequencing. Preferential cleavage: X-cleavage-Y-Z where X=any amino acid; Y=Leu, Phe, Ile, Val, Met, Ala and Z is any amino acid other than Pro. Cleavage N-terminal to Leu is preferred over cleavage of N-terminal to Phe which is preferred over the others. Often used to do limited proteolysis for peptide mapping and studies of protein structure and conformational changes.
Packaging
25 mg in poly bottle
1 g in glass bottle
100, 250 mg in glass bottle
Quality
Contains many extraneous enzymes.
Unit Definition
One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).
Physical form
lyophilized powder containing calcium and sodium acetate buffer salts
Preparation Note
The pH optimum is 8.0 and the optimal temperature for activity is 70 °C. Considerably stable from pH 5 to 9.5. Thermolysin has a low cleavage specificity, therefore, it produces a number of short fragments that are suitable for sequencing. Preferential cleavage: X-cleavage-Y-Z where X=any amino acid; Y=Leu, Phe, Ile, Val, Met, Ala and Z is any amino acid other than Pro. Cleavage N-terminal to Leu is preferred over cleavage of N-terminal to Phe which is preferred over the others.

type	Type X
Quality Level	200,
form	lyophilized powder
specific activity	30-350 units/mg protein (E1%/280)
mol wt	34.6 kDa by amino acid sequence
purified by	crystallization
shipped in	wet ice
storage temp.	20°C

pictograms	GHS07,GHS08
signalword	Danger
hcodes	H315 - H317 - H319 - H334 - H335
pcodes	P261 - P280 - P305 + P351 + P338 - P342 + P311
Target Organs	Respiratory system
Personal Protective Equipment	dust mask type N95 (US),, Eyeshields,, Faceshields,, Gloves,
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable