Anti-Guinea Pig IgG (whole molecule)Peroxidase antibody produced in rabbit A5545-1ML

MDL number:	MFCD00162368
NACRES:	NA.46

An immunoglobulin has two heavy chains and two light chains connected by disulfide bond. It is a glycoprotein. IgG is a major class of immunoglobulin. It has two subclasses of IgG- IgG1 and IgG2.
Specificity
Rabbit anti-guinea pig IgG (whole molecule)-peroxidase antibody binds to guinea pig IgG.
guinea pig IgG
Anti-Guinea Pig IgG (whole molecule) Peroxidase antibody produced in rabbit has been used in diphtheria serology enzyme-linked immunosorbent assay (ELISA) and immunoblotting.
Biochem/physiol Actions
IgGs are glycoprotein antibodies that modulate several immune responses. Guinea pig IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-guinea pig IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins.
Immunoglobulin G (IgG) is the major class and most abundant immunoglobulin of immune system released by plasma cells. IgG mediate immune response with respect to the type of antigen and is found in blood or extracellular fluid. IgG defends against bacterial and viral infections. Maternal IgG crosses from mother to fetus through the placental barrier by pinocytosis to provide passive immunity for the neonate and protect against infections.
Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. IgG1 helps in complement fixation through alternate pathway and IgG2 fixes complement by conventional mechanism. It mainly helps in immune defense. Guinea Pig is a highly preferred laboratory animal to study the local and anaphylactic reactions.
Physical form
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT
Preparation Note
Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).
Storage and Stability
Store at -20 °C for long term use. For continuous use, the product may be stored at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots at -20 °C. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	200,
antibody form	affinity isolated antibody
antibody product type	secondary antibodies
clone	polyclonal
form	buffered aqueous solution
species reactivity	guinea pig
application(s)	direct ELISA: 1:50,000-1:80,000, dot blot: 1:50,000-1:100,000 (chemiluminescent), immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400-1:800
conjugate	peroxidase conjugate
shipped in	dry ice
storage temp.	−20°C

pictograms	GHS07
signalword	Warning
hcodes	H317
pcodes	P280 - P302 + P352
RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	Not applicable
Flash Point C	Not applicable