Anti-Horse IgG (whole molecule)Peroxidase antibody produced in rabbit A9292-2ML

MDL number:	MFCD00162385
NACRES:	NA.46

Horse IgGs have seven subclasses ranging from IgG1 to IgG7. Equine IgG antibodies mainly regulate mucosal and systemic immunological responses and thereby, provide protection against disease-causing pathogens such as Streptococcus equi, and the horse flu virus. Horse IgG may also function to control the advancement of EHV-1 infection . Anti-horse IgG (whole molecule)peroxidase antibody is specific for IgG in horses.
Horse IgG
The level of S2-specific antibodies in test samples of horse serum was determined by ELISA using HRP-conjugated rabbit anti-horse IgG at a 1:35000 dilution with incubation for 45 minutes at room temperature.
Anti-horse IgG (whole molecule)peroxidase antibody may be used in dot blot (1:8,000-1:10,000), immunohistochemistry (formalin-fixed, paraffin-embedded sections, at 1:150 dilution) and direct ELISA (1:15,000).
Biochem/physiol Actions
IgG, a monoclonal antibody can be cleaved at the hinge region by nonspecific proteases like papain and pepsin. This can result in univalent Fab (antigen-binding fragments) fragments or bivalent F(ab′)2 fragments. These two enzymes have a broad substrate specificity resulting in heterogenous fragments.
Physical form
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT
Preparation Note
Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	200,
antibody form	IgG fraction of antiserum
antibody product type	secondary antibodies
clone	polyclonal
form	buffered aqueous solution
application(s)	direct ELISA: 1:15,000, dot blot: 1:100,000-1:200,000 (chemiluminescent), immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:150
conjugate	peroxidase conjugate
shipped in	dry ice
storage temp.	−20°C

pictograms	GHS08
signalword	Danger
hcodes	H334
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable