Anti-Human IgM (-chain specific)Peroxidase antibody produced in goat A0420-1ML

MDL number:	MFCD00162464
NACRES:	NA.46

B cells predominantly secrete IgM antibodies as pentamers, but may also secrete them as hexamers which are capable of activating the complement cascade more efficiently.
Goat anti-human IgM (mu-chain specific)-peroxidase antibody is specific for human IgM and does not bind to other human Igs. Furthermore, the antibody does not react with mouse and rat IgG. Hence, the antibody conjugate yields reduced background with mouse or rat samples.
human IgM
Goat anti-human IgM (mu-chain specific)-peroxidase antibody can be used:

• for dot blot (1:100,000) and immunohistochemistry (1:200 using formalin-fixed, paraffin-embedded sections) applications.
• in immunoblotting
• sandwich immunoassay

Anti- Human IgM was used in an ELISA assay to determine the concentration of IgM secreted by CH12 B lymphoma cells stimulated by LPS.
Biochem/physiol Actions
IgM plays a key role in engulfing of the apoptotic cells. A reduction in serum IgM levels leads to increased autoimmune response and higher risk for infections. IgM displays polyreactive and autoreactive functionality. It plays a key role in tissue homeostasis by mediating clearance of tissue based molecules.
Physical form
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT
Preparation Note
Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	goat
Quality Level	300,
antibody form	affinity isolated antibody
antibody product type	secondary antibodies
clone	polyclonal
species reactivity	human
application(s)	direct ELISA: 1:50,000, dot blot: 1:100,000 (chemiluminescent), immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200
conjugate	peroxidase conjugate
shipped in	dry ice
storage temp.	−20°C

pictograms	GHS07
signalword	Warning
hcodes	H317
pcodes	P280 - P302 + P352
Personal Protective Equipment	Eyeshields,, Gloves,
RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	Not applicable
Flash Point C	Not applicable