Anti-Mouse IgG (whole molecule)Alkaline Phosphatase antibody produced in rabbit A2418-1ML

MDL number:

MFCD00162640

IgG (immunoglobulin G) antibody subtype is a predominant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3.
purified mouse IgG
Anti-Mouse IgG (whole molecule) Alkaline Phosphatase antibody produced in rabbit has been used in :

• direct enzyme linked immunosorbent assay (ELISA)
• immunoblotting
• immunohistochemistry

Western blot analysis of lysates from the placental cell line 3ASubE-P-3 and U037 cells were performed using alkaline phosphatase conjugated rabbit anti- mouse IgG as the secondary at 1:2000 to recognize the mouse mom Il-8RB. Secondary was developed using bromochloroindoyl phosphate (Sigma) pH. 9.5 for 10-15 minutes.Western blot analysis of coimmunoprecipitates from human cell or rat cell vaginal extracts were performed using alkaline phosphatase conjugated rabbit anti- mouse IgG as the secondary at 1:15000 dilution.
Biochem/physiol Actions
Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. IgG antibody participates in complement fixation and opsonization.
Physical form
Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 and 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	200,
antibody form	IgG fraction of antiserum
antibody product type	secondary antibodies
clone	polyclonal
form	buffered aqueous solution
species reactivity	mouse
application(s)	direct ELISA: 1:15,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40 using human tonsil tissue, western blot (chemiluminescent): 1:40,000-1:80,000 using deecting β-Actin in total cell extract of HeLa cells (5-10 μg/mL)
conjugate	alkaline phosphatase conjugate
shipped in	wet ice
storage temp.	2-8°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable