Rabbit Anti-Sheep IgG Antibody, biotin-SP conjugate AP147B

eCl@ss:	32160702
NACRES:	NA.46

Specificity
Based on immunoelectrophoresis, the antibody reacts with the heavy chains on sheep IgG and with light chains common to most sheep immunoglobulins. No antibody was detected against non-immunoglobulin serum protein, but the antibody may cross-react with immunoglobulins from other species.
Purified Sheep IgG.
Rabbit anti-Sheep IgG Antibody, biotin-SP conjugate detects level of Sheep IgG & has been published & validated for use in ELISA & WB.
Research Category
Secondary & Control Antibodies
DILUTION:
1:50,000 - 1:1,000,000 for ELISA using enzyme-conjugated streptavidin
1:10,000 - 1:100,000 for Western Blots using enzyme-conjugated streptavidin
1:500 - 1:5,000 for enzyme immunohistochemistry on tissue sections
1:200 - 1:1,000 for flow cytometry and fluorescence immunohisto/cytochemistry
Optimal working dilution must be determined by the end user.
Research Sub Category
Whole Immunoglobulin Secondary Antibodies
Physical form
ImmunoAffinity Purified
Lyophilized. Buffer = 0.01M Phosphate buffer, 0.25 M NaCl with 15 mg/mL BSA and 0.05% sodium azide.
Storage and Stability
Maintain lyophilized product at 2-8°C for up to 12 months from date of receipt. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles. Expiration date: one year from date of reconstitution.
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	100,
antibody form	F(ab)2 fragment of affinity isolated antibody
antibody product type	secondary antibodies
clone	polyclonal
species reactivity	sheep
Торговая марка	Chemicon®
application(s)	ELISA: suitable, western blot: suitable
conjugate	biotin conjugate
shipped in	wet ice

hcodes	H412
pcodes	P273 - P501
WGK Germany	WGK 3