Sheep Anti-Mouse IgG Antibody, Species absorbed, Cy3 conjugate AC111C

eCl@ss:	32160702
NACRES:	NA.46

Immunoglobulin G (IgG) is part of the family of glycoprotein immunoglobulins which stage immune responses against pathogenic organisms. IgG contributes to the immune response by mobilizing innate immune effector cells and inducing and pro-inflammatory responses. Such proinflammatory responses are mediated by Fcg receptors which interact with sialylated Fc fragments on IgG molecules.
Specificity
Based on ELISA analysis using the unconjugated antibody (Cat. No. AC111), this antibody reacts with mouse IgG whole molecule (IgG>>IgG2a>IgG>>>>IgG1) with minimal or no cross-reactivity toward mouse IgA or IgM.
This antibody has been species absorbed and tested by ELISA prior to conjugation to ensure minimal cross-reactivity to chicken IgY or IgG from human, rat, rabbit, guinea pig, and hamster species, but may cross-react with immunoglobulins from other species.
Purified mouse IgG.
Research Sub Category
Fragment Specific Secondary Antibodies
Research Category
Secondary & Control Antibodies
Sheep Anti-Mouse IgG Antibody, Species absorbed, Cy3 conjugate is an antibody against Mouse IgG for use in Immunocytochemistry.
Immunocytochemistry Analysis: A representative lot was validated by indirect fluorescent labelling of trimethylated histone H3 in HeLa and NIH/3T3 cells following a mouse monoclonal primary antibody (Cat. No. 05-1951) in immunocytochemistry application.
Quality
Evaluated by Immunocytochemistry in A431.
Immunocytochemistry Analysis: Performance of this Cy3-conjugated secondary antibody is validated by indirect fluorescent labelling of trimethylated histone H3 in A431 following a mouse monoclonal primary antibody (Cat. No. 05-1951) in immunocytochemistry application.
Target description
~150 kDa observed.
Physical form
Purified sheep polyclonal antibody in PBS with 15 mg/mL BSA and 0.1 % sodium azide.
Fractionated by octanoic acid and ammonium sulfate cut and then depleted with rat IgG column and mouse IgA column prior to Cy3 conjugation.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	sheep
Quality Level	100,
antibody form	purified antibody
antibody product type	secondary antibodies
clone	polyclonal
species reactivity	mouse
application(s)	immunocytochemistry: suitable
conjugate	CY3 conjugate
shipped in	wet ice

WGK Germany	WGK 2
Flash Point F	Not applicable
Flash Point C	Not applicable