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UKKi029-A 66540872-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Klinikum der Universitat zu KolnCell Line DescriptionDerivation
Primary cell type: Peripheral blood mononuclear cell
Tissue collection year: 2017
Passage number reprogrammed: 0
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: No
Merthods used: immune_staining, pcr
Notes on reprogramming vector detection: sendai virus absent
Files showing reprogramming vector expressed or silenced: Image / Result
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
SSEA-4 Yes - -
TRA 1-80 Yes -
SOX2 Yes -
OCT 4 Yes - -
NANOG Yes - -
FOXD3 Yes -
DNMT3b Yes -
Morphology:
Differentiation potency
Ectoderm:
Ectoderm
In vitro directed differentiation
Marker Expressed
Nestin Yes
Morphology:
Endoderm:
Endoderm
In vitro directed differentiation
Marker Expressed
SOX17 Yes
Morphology:
Mesoderm:
Mesoderm
In vitro directed differentiation
Marker Expressed
BRACHYURY Yes
Morphology:
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Genotyping
Karyotyping
Passage number: 18
Cell line karyotype: No larger chromosomal aberrations to be reported.
Karyotyping method: Molecular karyotyping by SNP array
Image / ResultLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Vitronectin
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: other
Culture base medium: StemFlex™
Main protein source: Albumine
Passage Number banked (pre-EBiSC): 16
No. Vials banked (pre-EBiSC): 25Legal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
StemFlex is a trademark of Life Technologies CorporationПараметрыbiological source human peripheral blood reprogramming method Sendai virus description age (20-24) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) prolonged QT interval shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UKKi029-B 66540873-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Klinikum der Universitat zu KolnCell Line DescriptionDerivation
Primary cell type: Peripheral blood mononuclear cell
Tissue collection year: 2017
Passage number reprogrammed: 0
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Files showing reprogramming vector expressed or silenced: Image / Result
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
TRA 1-80 Yes - -
FOXD3 Yes -
OCT 4 Yes - -
NANOG Yes - -
DNMT3b Yes -
SOX2 Yes -
SSEA-1 Yes -
SSEA-4 Yes - -
Morphology:
Morphology pictures of the Masterbank and the thawing control do
Morphology pictures of the thawing control d1 and d2
Morphology pictures of the thawing control d3
Differentiation potency
Ectoderm:
Ectoderm
In vitro directed differentiation
Marker Expressed
Nestin Yes
Morphology:
Endoderm:
Endoderm
In vitro directed differentiation
Marker Expressed
SOX17 Yes
Morphology:
Mesoderm:
Mesoderm
In vitro directed differentiation
Marker Expressed
BRACHYURY Yes
Morphology:
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Genotyping
Karyotyping
Passage number: 17
Cell line karyotype: No larger chromosomal aberrations to be reported.
Karyotyping method: Molecular karyotyping by SNP array
Image / ResultLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Vitronectin
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: other
Culture base medium: StemFlex™
Main protein source: Albumine
Passage Number banked (pre-EBiSC): 19
No. Vials banked (pre-EBiSC): 25Legal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
StemFlex is a trademark of Life Technologies CorporationПараметрыbiological source human peripheral blood reprogramming method Sendai virus description age (20-24) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) prolonged QT interval shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UKKi029-C 66540874-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Klinikum der Universitat zu KolnCell Line DescriptionDerivation
Primary cell type: Peripheral blood mononuclear cell
Tissue collection year: 2017
Passage number reprogrammed: 0
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Files showing reprogramming vector expressed or silenced: Image / Result
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
SSEA-4 Yes - -
SSEA-1 Yes -
FOXD3 Yes -
TRA 1-80 Yes - -
OCT 4 Yes - -
SOX2 Yes -
NANOG Yes - -
DNMT3b Yes -
Morphology:
Differentiation potency
Ectoderm:
Ectoderm
In vitro directed differentiation
Marker Expressed
Nestin Yes
Morphology:
Endoderm:
Endoderm
In vitro directed differentiation
Marker Expressed
SOX17 Yes
Morphology:
Protocol or reference:
STEMdiff_Trilienage_protocol.pdf
Mesoderm:
Mesoderm
In vitro directed differentiation
Marker Expressed
BRACHYURY Yes
Morphology:
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Genotyping
Karyotyping
Passage number: 21
Cell line karyotype: No larger chromosomal aberrations to be reported.
Karyotyping method: Molecular karyotyping by SNP array
Image / ResultLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Vitronectin
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: other
Culture base medium: StemFlex™
Main protein source: Albumine
Passage Number banked (pre-EBiSC): 19
No. Vials banked (pre-EBiSC): 25Legal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
StemFlex is a trademark of Life Technologies CorporationПараметрыbiological source human peripheral blood reprogramming method Sendai virus description age (20-24) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) prolonged QT interval shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UKKi030-A 66540875-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Klinikum der Universitat zu KolnCell Line DescriptionDerivation
Primary cell type: Peripheral blood mononuclear cell
Tissue collection year: 2016
Passage number reprogrammed: 0
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Files showing reprogramming vector expressed or silenced: Image / Result
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG Yes - -
FOXD3 Yes -
DNMT3b Yes -
TRA 1-80 Yes -
SSEA-4 Yes - -
OCT4 Yes - -
SOX2 Yes -
Morphology:
Differentiation potency
Ectoderm:
Ectoderm
In vitro directed differentiation
Marker Expressed
Nestin Yes
Morphology:
Endoderm:
Endoderm
In vitro directed differentiation
Marker Expressed
SOX17 Yes
Morphology:
Protocol or reference:
STEMdiff_Trilineage_protocol.pdf
Mesoderm:
Mesoderm
In vitro directed differentiation
Marker Expressed
BRACHYURY Yes
Morphology:
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Genotyping
Karyotyping
Passage number: 15
Cell line karyotype: No larger chromosomal aberrations to be reported.
Karyotyping method: Molecular karyotyping by SNP array
Image / ResultLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Vitronectin
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: other
Culture base medium: StemFlex™
Main protein source: Albumine
Passage Number banked (pre-EBiSC): 18
No. Vials banked (pre-EBiSC): 20Legal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
StemFlex is a trademark of Life Technologies CorporationПараметрыbiological source human peripheral blood reprogramming method Sendai virus description age (65-69) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) Brugada syndrome shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UKKi030-B 66540876-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Klinikum der Universitat zu KolnCell Line DescriptionDerivation
Primary cell type: Peripheral blood mononuclear cell
Tissue collection year: 2016
Passage number reprogrammed: 0
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: No
Merthods used: immune_staining, pcr
Files showing reprogramming vector expressed or silenced: Image / Result
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
DNMT3b Yes -
FOXD3 No -
SSEA-4 Yes - -
OCT 4 Yes - -
SOX2 No -
SSEA-1 Yes -
NANOG Yes - -
TRA 1-80 Yes - -
Morphology:
Differentiation potency
Ectoderm:
Ectoderm
In vitro directed differentiation
Marker Expressed
Nestin Yes
Morphology:
p24
Endoderm:
Endoderm
In vitro directed differentiation
Marker Expressed
SOX17 Yes
Morphology:
p24
Mesoderm:
Mesoderm
In vitro directed differentiation
Marker Expressed
BRACHYURY Yes
Morphology:
p24
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Genotyping
Karyotyping
Passage number: 18
Cell line karyotype: No larger chromosomal aberrations to be reported.
Karyotyping method: Molecular karyotyping by SNP array
Image / ResultLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Vitronectin
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: other
Culture base medium: STEMFLEX™
Main protein source: Albumine
Passage Number banked (pre-EBiSC): 20
No. Vials banked (pre-EBiSC): 25Legal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
StemFlex is a trademark of Life Technologies CorporationПараметрыbiological source human peripheral blood reprogramming method Sendai virus description age (N/A) Торговая марка EBiSC™ gender N/A growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) Brugada syndrome shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UKKi030-C 66540877-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Klinikum der Universitat zu KolnCell Line DescriptionDerivation
Primary cell type: Peripheral blood mononuclear cell
Tissue collection year: 2016
Passage number reprogrammed: 0
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Files showing reprogramming vector expressed or silenced: Image / Result
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
SOX2 Yes -
TRA 1-80 Yes -
OCT 4 Yes - -
SSEA-4 Yes - -
NANOG Yes - -
DNMT3b Yes -
FOXD3 Yes -
Morphology:
Differentiation potency
Ectoderm:
Ectoderm
In vitro directed differentiation
Marker Expressed
Nestin Unknown
Morphology:
Endoderm:
Endoderm
In vitro directed differentiation
Marker Expressed
SOX17 Yes
Morphology:
Protocol or reference:
STEMdiff_Trilineage_protocol.pdf
Mesoderm:
Mesoderm
In vitro directed differentiation
Marker Expressed
BRACHYURY Yes
Morphology:
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Genotyping
Karyotyping
Passage number: 18
Cell line karyotype: No larger chromosomal aberrations to be reported.
Karyotyping method: Molecular karyotyping by SNP array
Image / ResultLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Vitronectin
Passage method: Enzyme-free cell dissociation
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: other
Culture base medium: StemFlex™
Main protein source: Albumine
Passage Number banked (pre-EBiSC): 17
No. Vials banked (pre-EBiSC): 20Legal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
StemFlex is a trademark of Life Technologies CorporationПараметрыbiological source human peripheral blood reprogramming method Sendai virus description age (N/A) Торговая марка EBiSC™ gender N/A growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) Brugada syndrome shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UKKi031-A 66540878-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Klinikum der Universitat zu KolnCell Line DescriptionDerivation
Primary cell type: Peripheral blood mononuclear cell
Tissue collection year: 2016
Passage number reprogrammed: 0
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Files showing reprogramming vector expressed or silenced: Image / Result
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
DNMT3b Yes -
SSEA-4 Yes - -
SOX2 Yes -
TRA 1-80 Yes -
OCT 4 Yes - -
FOXD3 Yes -
NANOG Yes - -
Differentiation potency
Ectoderm:
Ectoderm
In vitro directed differentiation
Marker Expressed
Nestin Yes
Morphology:
Endoderm:
Endoderm
In vitro directed differentiation
Marker Expressed
SOX17 Yes
Morphology:
Mesoderm:
Mesoderm
In vitro directed differentiation
Marker Expressed
BRACHYURY Yes
Morphology:
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Genotyping
Karyotyping
Passage number: 16
Cell line karyotype: No larger chromosomal aberrations to be reported. Chr20: Duplication in q11.21: Cell culture adaptation.
Karyotyping method: Molecular karyotyping by SNP array
Image / ResultLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Vitronectin
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: other
Culture base medium: StemFlex™
Main protein source: Albumine
Passage Number banked (pre-EBiSC): 19
No. Vials banked (pre-EBiSC): 25Legal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
StemFlex is a trademark of Life Technologies CorporationПараметрыbiological source human peripheral blood reprogramming method Sendai virus description age (55-59) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) hypertrophic cardiomyopathy shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UKKi031-B 66540879-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Klinikum der Universitat zu KolnCell Line DescriptionDerivation
Primary cell type: Peripheral blood mononuclear cell
Tissue collection year: 2016
Passage number reprogrammed: 0
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Files showing reprogramming vector expressed or silenced: Image / Result
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
SOX2 Yes -
OCT 4 Yes - -
NANOG Yes - -
TRA 1-80 Yes -
SSEA-4 Yes - -
DNMT3b Yes -
FOXD3 Yes -
Morphology:
Differentiation potency
Ectoderm:
Ectoderm
In vitro directed differentiation
Morphology:
Endoderm:
Endoderm
In vitro directed differentiation
Marker Expressed
SOX17 Yes
Morphology:
Mesoderm:
Mesoderm
In vitro directed differentiation
Marker Expressed
BRACHYURY Yes
Morphology:
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Genotyping
Karyotyping
Passage number: 18
Cell line karyotype: No larger chromosomal aberrations to be reported; Chr20: Duplication in q11.21.
Karyotyping method: Molecular karyotyping by SNP array
Image / ResultLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Vitronectin
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: other
Culture base medium: StemFlex™
Main protein source: Albumine
Passage Number banked (pre-EBiSC): 21
No. Vials banked (pre-EBiSC): 25Legal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
StemFlex is a trademark of Life Technologies CorporationПараметрыbiological source human peripheral blood reprogramming method Sendai virus description age (60-64) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) hypertrophic cardiomyopathy shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UKKi031-C 66540880-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Klinikum der Universitat zu KolnCell Line DescriptionDerivation
Primary cell type: Peripheral blood mononuclear cell
Passage number reprogrammed: 0
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Files showing reprogramming vector expressed or silenced: Image / Result
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
SOX2 Yes -
SSEA-4 Yes - -
DNMT3b Yes -
TRA 1-80 Yes -
NANOG Yes - -
OCT 4 Yes - -
FOXD3 Yes -
Morphology:
Differentiation potency
Ectoderm:
Ectoderm
In vitro directed differentiation
Marker Expressed
Nestin Unknown
Morphology:
Endoderm:
Endoderm
In vitro directed differentiation
Marker Expressed
SOX17 Yes
Morphology:
Mesoderm:
Mesoderm
In vitro directed differentiation
Marker Expressed
BRACHYURY Yes
Morphology:
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Genotyping
Karyotyping
Passage number: 15
Cell line karyotype: No larger chromosomal aberrations to be reported; Chr20: Duplication in q11.21
Karyotyping method: Molecular karyotyping by SNP array
Image / ResultLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Vitronectin
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: other
Culture base medium: StemFlex™
Main protein source: Albumine
Passage Number banked (pre-EBiSC): 18
No. Vials banked (pre-EBiSC): 25Legal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
StemFlex is a trademark of Life Technologies CorporationПараметрыbiological source human peripheral blood reprogramming method Sendai virus description age (N/A) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) hypertrophic cardiomyopathy shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UKKi032-A 66540881-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Klinikum der Universitat zu KolnCell Line DescriptionDerivation
Primary cell type: Peripheral blood mononuclear cell
Tissue collection year: 2016
Passage number reprogrammed: 0
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Files showing reprogramming vector expressed or silenced: Image / Result
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
OCT 4 Yes - -
FOXD3 Yes -
NANOG Yes - -
SOX2 Yes -
SSEA-4 Yes - -
TRA1-80 Yes -
DNMT3b Yes -
Morphology:
Differentiation potency
Ectoderm:
Ectoderm
In vitro directed differentiation
Marker Expressed
Nestin Yes
Morphology:
Endoderm:
Endoderm
In vitro directed differentiation
Marker Expressed
SOX17 Yes
Morphology:
Mesoderm:
Mesoderm
In vitro directed differentiation
Marker Expressed
BRACHYURY Yes
Morphology:
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Genotyping
Karyotyping
Passage number: 22
Cell line karyotype: No larger chromosomal aberrations to be reported; Chr12: Duplication of telomeric
Image / ResultLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Vitronectin
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: other
Culture base medium: StemFlex™
Main protein source: -
Passage Number banked (pre-EBiSC): 23
No. Vials banked (pre-EBiSC): 25Legal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
StemFlex is a trademark of Life Technologies CorporationПараметрыbiological source human peripheral blood reprogramming method Sendai virus description age (40-44) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) none shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UKKi032-B 66540882-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Klinikum der Universitat zu KolnCell Line DescriptionDerivation
Primary cell type: Peripheral blood mononuclear cell
Tissue collection year: 2016
Passage number reprogrammed: 0
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Files showing reprogramming vector expressed or silenced: Image / Result
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG Yes - -
SSEA-4 Yes - -
SSEA-1 Yes -
SOX2 Yes -
FOXD3 Yes -
TRA 1-81 Yes - -
OCT4 Yes - -
DNMT3b Yes -
Morphology:
Differentiation potency
Ectoderm:
Ectoderm
In vitro directed differentiation
Marker Expressed
Nestin Yes
Morphology:
Endoderm:
Endoderm
In vitro directed differentiation
Marker Expressed
SOX17 Yes
Morphology:
Mesoderm:
Mesoderm
In vitro directed differentiation
Marker Expressed
BRACHYURY Yes
Morphology:
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Genotyping
Karyotyping
Passage number: 23
Cell line karyotype: No larger chromosomal aberrations to be reported; Chr12: Duplication of telomeric
Karyotyping method: Molecular karyotyping by SNP array
Image / ResultLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Vitronectin
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: other
Culture base medium: StemFlex™
Main protein source: -
Passage Number banked (pre-EBiSC): 20
No. Vials banked (pre-EBiSC): 21Legal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
StemFlex is a trademark of Life Technologies CorporationПараметрыbiological source human peripheral blood reprogramming method Sendai virus description age (40-44) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) none shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UKKi032-C 66540883-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Klinikum der Universitat zu KolnCell Line DescriptionDerivation
Primary cell type: Peripheral blood mononuclear cell
Tissue collection year: 2016
Passage number reprogrammed: 0
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Files showing reprogramming vector expressed or silenced: Image / Result
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
DNMT3b Yes -
SOX2 Yes -
TRA 1-80 Yes - -
SSEA-1 Yes -
SSEA-4 Yes - -
NANOG Yes - -
FOXD3 Yes -
OCT 4 Yes - -
Morphology:
Differentiation potency
Ectoderm:
Ectoderm
In vitro directed differentiation
Marker Expressed
Nestin Yes
Morphology:
Endoderm:
Endoderm
In vitro directed differentiation
Marker Expressed
SOX17 Yes
Morphology:
Mesoderm:
Mesoderm
In vitro directed differentiation
Marker Expressed
BRACHYURY Yes
Morphology:
Protocol or reference:
STEMdiff_Trilineage_protocol.pdf
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Genotyping
Karyotyping
Passage number: 21
Cell line karyotype: No larger chromosomal aberrations to be reported.
Image / ResultLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Vitronectin
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: other
Culture base medium: StemFlex™
Main protein source: -
Passage Number banked (pre-EBiSC): 22
No. Vials banked (pre-EBiSC): 25Legal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
StemFlex is a trademark of Life Technologies CorporationПараметрыbiological source human peripheral blood reprogramming method Sendai virus description age (40-44) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) none shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UNEWi001-A 66540039-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of NewcastleCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Primary cell developmental stage: Adult
Location of primary tissue procurement: University of Newcastle, Biobank
Tissue collection date: June 24, 2013
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown
Characterization
Differentiation potency
Ectoderm: In vitro spontaneous differentiation
Endoderm: In vitro spontaneous differentiation
Mesoderm: In vitro spontaneous differentiation
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: -
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 21%
Temperature: 37°CLegal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) Quality Level 100, reprogramming method Sendai virus description age (45-49) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) retinitis pigmentosa shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UNEWi002-A 66540040-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of NewcastleCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Primary cell developmental stage: Adult
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown
Characterization
Analysis of Undifferentiated Cells
Marker expression: SSEA-4(+)TRA 1-60(+)SSEA-1 (+)NANOG(+)
Differentiation potency
Ectoderm: In vitro spontaneous differentiation
Endoderm: In vitro spontaneous differentiation
Mesoderm: In vitro spontaneous differentiation
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: -
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Cell line karyotype: CytoSNP
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 21%
Temperature: 37°CLegal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) Quality Level 100, reprogramming method Sendai virus description age (50-54) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) retinitis pigmentosa shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UNEWi004-A 66540017-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of NewcastleCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Primary cell developmental stage: Adult
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown
Derivation
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression: NANOG(+)SSEA-4(+)TRA 1-60( +)
Differentiation potency
Ectoderm: In vitro spontaneous differentiation
Endoderm: In vitro spontaneous differentiation
Mesoderm: In vitro spontaneous differentiation
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: -
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Cell line karyotype: CytoSNP
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 21%
Temperature: 37°CLegal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) reprogramming method Sendai virus description age (45-49) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) retinitis pigmentosa shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UNEWi005-A 66540020-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of NewcastleCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Primary cell developmental stage: Adult
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown
Characterization
Analysis of Undifferentiated Cells
Marker expression: SSEA-4(+)TRA 1-60(+)NANOG (+)
Differentiation potency
Ectoderm: In vitro spontaneous differentiation
Endoderm: In vitro spontaneous differentiation
Mesoderm: In vitro spontaneous differentiation
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: -
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Cell line karyotype: CytoSNP
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 21%
Temperature: 37°CLegal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) reprogramming method Sendai virus description age (50-54) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) retinitis pigmentosa shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UNEWi017-A 66540057-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of NewcastleCell Line DescriptionDerivation
Primary cell type: Fibroblast
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown
Characterization
Analysis of Undifferentiated Cells
Marker expression: TRA 1-60(+)SSEA-4 (+)SSEA-1(-)POU5F1 (OCT-4)(+)NANOG(+)
hPSC Scorecard: Positive Negative,Self-renewal,Endoderm,Mesoderm,Ectoderm
Differentiation potency
Ectoderm: In vivo teratoma,In vitro spontaneous differentiation
Endoderm: In vivo teratoma,In vitro spontaneous differentiation
Mesoderm: In vivo teratoma,In vitro spontaneous differentiation
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Cell line karyotype: two small regions of homozygosity
Karyotyping method: CytoSNP
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 21%
Temperature: 37°CLegal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) reprogramming method Sendai virus description age (15-19) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) aplastic anemia shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UNEWi018-A 66540012-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of NewcastleCell Line DescriptionDerivation
Primary cell type: Fibroblast
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown
Characterization
Analysis of Undifferentiated Cells
Marker expression: TRA 1-60(+)SSEA-1(-)POU5F1 (OCT-4)(+)SSEA-4(+)
Differentiation potency
Ectoderm: In vivo teratoma,In vitro spontaneous differentiation
Endoderm: In vivo teratoma,In vitro spontaneous differentiation
Mesoderm: In vivo teratoma,In vitro spontaneous differentiation
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Cell line karyotype: trisomy chromosome 3
Karyotyping method: CytoSNP
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 21%
Temperature: 37°CLegal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) Quality Level 100, reprogramming method Sendai virus description age (10-14) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) aplastic anemia shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UNEWi019-A 66540021-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of NewcastleCell Line DescriptionDerivation
Primary cell type: Fibroblast
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown
Characterization
Analysis of Undifferentiated Cells
Marker expression: NANOG(+),SSEA-1 (-),TRA 1-60(+),SSEA-4 (+)
Differentiation potency
Ectoderm: In vitro spontaneous differentiation
Endoderm: In vitro spontaneous differentiation
Mesoderm: In vitro spontaneous differentiation
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Cell line karyotype: no clinical significant imbalance was detected
Karyotyping method: Molecular karyotyping by SNP array
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 21%
Temperature: 37°CLegal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) reprogramming method Sendai virus description age (20-24) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) anemia shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UNEWi021-A 66540174-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of NewcastleCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Primary cell developmental stage: Adult
Passage number reprogrammed: P4
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown
Other:From the same donor: UNEWi021-B, UNEWi021-C
Characterization
Analysis of Undifferentiated Cells
Marker expression: NANOG(+)SSEA-4 (+)SSEA1 (-)TRA 1-60(+)
Differentiation potency
Ectoderm:
Retinal Pigment Epithelium
In vitro spontaneous differentiation
In vitro directed differentiation
Marker Expressed:Tuj1 (+)
Endoderm: In vitro spontaneous differentiation
Marker Expressed:AFP (+)
Mesoderm: In vitro spontaneous differentiation
Marker Expressed:SMA(+)
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: P10
Cell line karyotype: No clinically significant imbalance was detected
Karyotyping method: Molecular karyotyping by SNP array
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 20%
Temperature: 37°CLegal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) reprogramming method Sendai virus description age (80-84) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) none shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UNEWi021-B 66540175-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of NewcastleCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Primary cell developmental stage: Adult
Passage number reprogrammed: P4
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown
Other:From the same donor: UNEWi021-A, UNEWi021-C
Characterization
Analysis of Undifferentiated Cells
Marker expression: SSEA-4(+)SSEA1(-) TRA 1-60(+)NANOG (+)
Differentiation potency
Ectoderm: In vitro spontaneous differentiation
Marker Expressed:TUBB3 (+)
Endoderm: In vitro spontaneous differentiation
Marker Expressed:AFP(+)
Mesoderm: In vitro spontaneous differentiation
Marker Expressed:ACTA2 (+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: P12
Cell line karyotype: No clinically significant imbalance was detected
Karyotyping method: Molecular karyotyping by SNP array
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 20%
Temperature: 37°CLegal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) reprogramming method Sendai virus description age (80-84) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) none shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UNEWi021-C 66540176-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of NewcastleCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Primary cell developmental stage: Adult
Passage number reprogrammed: P4
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown
Other:From the same donor: UNEWi021-A,UNEWi021-B
Characterization
Analysis of Undifferentiated Cells
Marker expression: NANOG(+)SSEA1(-)TRA 1-60 (+)SSEA-4(+)
Differentiation potency
Ectoderm: In vitro spontaneous differentiation
Marker Expressed:TUBB3(+)
Endoderm: In vitro spontaneous differentiation
Marker Expressed:AFP(+)
Mesoderm: In vitro spontaneous differentiation
Marker Expressed:ACTA2 (+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: P12
Cell line karyotype: No clinically significant imbalance was detected
Karyotyping method: Molecular karyotyping by SNP array
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 20%
Temperature: 37°CLegal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) Quality Level 100, reprogramming method Sendai virus description age (80-84) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) none shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UNEWi022-A 66540177-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of NewcastleCell Line DescriptionDerivation
Primary Cell Type: Fibroblast of dermis
Developmental Stage: Adult
Passage Number: P4
Reprogramming Method: Sendai Virus (KLF4, MYC, POU5F1, SOX2)
Other: From the same donor as UNEWi022-B, UNEWi022-C
Characterization
Marker Expression: NANOG(+), TRA160(+), SSEA4(+), SSEA1(-)
Differentiation Potential: Ectoderm (TUBB3+), Endoderm (AFP+), Mesoderm (ACTA2+)
Microbiology / Virus Screening
HIV1: Negative
HIV2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Microbial Growth: None Detected
Viability: Viable Post-Cryopreservation
Genotyping
Karyotype: P14, Chromosome 12 gain by molecular karyotype SNP array
Genotype: 16 allele STR profile available upon requestLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: mTeSR®
Passage method: EDTA
Matrix: Matrigel®/Geltrex®
CO2 concentration: 5%
O2 concentration: 20%
Temperature: 37°CLegal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) reprogramming method Sendai virus description age (85-89) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) age-related macular degeneration shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UNEWi022-B 66540178-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of NewcastleCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Developmental stage: Adult
Passage number reprogrammed: P4
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list: KLF4,MYC,POU5F1,SOX2
Other: UNEWi022-A, UNEWi022-C
Characterization
Marker expression:
SSEA1(-),NANOG (+),SSEA-4 (+),TRA 1-60 (+)
Differentiation potency: Ectoderm TUBB3(+),Endoderm AFP (+),Mesoderm ACTA2 (+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: P14
Cell line karyotype: chromosome 12 gain
Karyotyping method: Molecular karyotyping by SNP array
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 20%
Temperature: 37°CLegal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) reprogramming method Sendai virus description age (85-89) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) age-related macular degeneration shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UNEWi022-C 66540179-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of NewcastleCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Developmental stage: Adult
Passage number reprogrammed: P4
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list: KLF4,MYC,POU5F1,SOX2
Other:From the same donor: UNEWi022-A, UNEWi022-B
Characterization
Marker expression:
SSEA1(-),NANOG(+),TRA 1-60(+),SSEA-4(+)
Differentiation potency
Ectoderm: TUBB3(+)Endoderm: AFP (+)Mesoderm: ACTA2 (+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: P14
Cell line karyotype: No clinically significant imbalance was detected
Karyotyping method: Molecular karyotyping by SNP array
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 20%
Temperature: 37°CLegal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) reprogramming method Sendai virus description age (85-89) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) age-related macular degeneration shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UNEWi023-A 66540441-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of NewcastleCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Primary cell developmental stage: Adult
Passage number reprogrammed: P4
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Other: From the same donor: UNEWi023-B, UNEWi023-C
Characterization
Marker expression: TRA 1-60 (+),NANOG (+),SSEA-1 (-),SSEA-4 (+)
Differentiation potency: Ectoderm: TUJ-1 (+),Endoderm: Alpha FetoProtein(+),Mesoderm: SMA (+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: P9
Cell line karyotype: lack of X chromosome detected
Karyotyping method: Molecular karyotyping by SNP array
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 20%
Temperature: 37°CLegal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) Quality Level 100, reprogramming method Sendai virus description age (85-89) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) age-related macular degeneration shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UNEWi023-B 66540442-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of NewcastleCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Primary cell developmental stage: Adult
Passage number reprogrammed: P4
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Other:From the same donor: UNEWi023-A, UNEWi023-C
Characterization
Marker expression: SSEA-4 (+),TRA 1-60 (+),SSEA-1 (-),NANOG (+)
Differentiation potency:Ectoderm: TUJ-1(+),Endoderm: Alpha FetoProtein (+),Mesoderm: SMA (+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: P9
Cell line karyotype: No clinically significant imbalance was detected
Karyotyping method: Molecular karyotyping by SNP array
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 20%
Temperature: 37°CLegal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) reprogramming method Sendai virus description age (85-89) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) age-related macular degeneration shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UNEWi023-C 66540443-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of NewcastleCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Primary cell developmental stage: Adult
Passage number reprogrammed: P4
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Other: From the same donor: UNEWi023-A, UNEWi023-B
Characterization
Marker expression:
NANOG (+) ,TRA 1-60 (+),SSEA-4(+),SSEA-1 (-)
Differentiation potency:Ectoderm: TUJ-1 (+),Endoderm: Alpha FetoProtein (+),Mesoderm: SMA (+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: P9
Cell line karyotype: No clinically significant imbalance was detected
Karyotyping method: Molecular karyotyping by SNP array
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Matrigel®/Geltrex®
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: mTeSR® 1
Passage Number banked (pre-EBiSC): P9
No. Vials banked (pre-EBiSC): 30Legal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) reprogramming method Sendai virus description age (85-89) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) age-related macular degeneration shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UNEWi024-A 66540323-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of NewcastleCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Developmental stage: Adult
Passage number reprogrammed: P4
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list: KLF4,MYC,POU5F1,SOX2
Other:From the same donor: UNEWi024-B, UNEWi024-C
Characterization
Marker expression: TRA 1-60(+),SSEA-4 (+),SSEA-1 (-),NANOG (+)
Differentiation potency: Ectoderm: TUBB3 (+),Endoderm: AFP (+),Mesoderm: ACTA2 (+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Cell line karyotype: No clinically significant imbalance was detected
Karyotyping method: Molecular karyotyping by SNP array
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 20%
Temperature: 37°CLegal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) reprogramming method Sendai virus description age (75-79) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) age-related macular degeneration shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UNEWi024-B 66540324-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of NewcastleCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Developmental stage: Adult
Passage number reprogrammed: P4
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list: KLF4,MYC,POU5F1,SOX2
Other: From the same donor: UNEWi024-A, UNEWi024-C
Characterization
Marker expression: TRA 1-60 (+),SSEA-4 (+),NANOG (+),SSEA-1 (-)
Differentiation potency Ectoderm: TUBB3 (+),Endoderm: AFP (+),Mesoderm: ACTA2 (+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: P13
Cell line karyotype: No clinically significant imbalance was detected
Karyotyping method: Molecular karyotyping by SNP array
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 20%
Temperature: 37°CLegal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) Quality Level 100, reprogramming method Sendai virus description age (75-79) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) age-related macular degeneration shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UNEWi024-C 66540325-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of NewcastleCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Developmental stage: Adult
Passage number reprogrammed: P4
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4,MYC,POU5F1,SOX2
Other: From the same donor: UNEWi024-A, UNEWi024-B
Characterization
Marker expression: SSEA-4 (+),NANOG (+),TRA 1-60 (+),SSEA-1 (-)
Differentiation potency: Ectoderm: TUBB3 (+),Endoderm: AFP (+),Mesoderm: ACTA2 (+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: P13
Cell line karyotype: No clinically significant imbalance was detected
Karyotyping method: Molecular karyotyping by SNP array
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 20%
Temperature: 37°CLegal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) Quality Level 100, reprogramming method Sendai virus description age (75-79) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) age-related macular degeneration shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UNEWi025-A 66540328-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of NewcastleCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Primary cell developmental stage: Adult
Passage number reprogrammed: P4
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list: KLF4,MYC,POU5F1,SOX2
Other: From the same donor: UNEWi025-B, UNEWi025-C
Characterization
Marker expression: NANOG (+),SSEA-4 (+),SSEA-1 (-),TRA 1-60 (+)
Differentiation potency: Ectoderm: TUBB3(+),Endoderm: AFP(+),Mesoderm: ACTA2 (+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: P12
Cell line karyotype: No clinically significant imbalance was detected
Karyotyping method: Molecular karyotyping by SNP array
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 20%
Temperature: 37°CLegal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) Quality Level 100, reprogramming method Sendai virus description age (70-74) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) age-related macular degeneration shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UNEWi025-B 66540329-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of NewcastleCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Primary cell developmental stage: Adult
Passage number reprogrammed: P4
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Other:From the same donor: UNEWi025-A, UNEWi025-C
Characterization
Marker expression:
SSEA-1 (-),SSEA-4 (+),TRA 1-60 (+),NANOG (+)
Differentiation potency: Ectoderm: TUBB3(+),Endoderm: AFP(+),Mesoderm: ACTA2 (+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: P12
Cell line karyotype: No clinically significant imbalance was detected
Karyotyping method: Molecular karyotyping by SNP array
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 20%
Temperature: 37°CLegal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) Quality Level 100, reprogramming method Sendai virus description age (70-74) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) age-related macular degeneration shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UNEWi025-C 66540330-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of NewcastleCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Primary cell developmental stage: Adult
Passage number reprogrammed: P4
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Other:From the same donor: UNEWi025-A, UNEWi025-B
Characterization
Marker expression: NANOG (+),SSEA-1 (-),SSEA-4 (+),TRA 1-60 (+)
Differentiation potency: Ectoderm: TUBB3 (+),Endoderm: AFP (+),Mesoderm: ACTA2 (+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: P12
Cell line karyotype: No clinically significant imbalance was detected
Karyotyping method: Molecular karyotyping by SNP array
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 20%
Temperature: 37°CLegal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) Quality Level 100, reprogramming method Sendai virus description age (70-74) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) age-related macular degeneration shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UNEWi026-A 66540444-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of NewcastleCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Primary cell developmental stage: Adult
Passage number reprogrammed: P4
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Other:From the same donor: UNEWi026-B, UNEWi026-C
Characterization
Marker expression: SSEA-1(-),TRA 1-60 (+),NANOG (+),SSEA-4 (+)
Differentiation potency:Ectoderm: TUJ-1(+),Endoderm: Alpha FetoProtein (+),Mesoderm: SMA (+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: P10
Cell line karyotype: No clinically significant imbalance was detected
Karyotyping method: Molecular karyotyping by SNP array
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 20%
Temperature: 37°CLegal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) Quality Level 100, reprogramming method Sendai virus description age (60-64) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) diabetes (mellitus) shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UNEWi026-B 66540445-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of NewcastleCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Primary cell developmental stage: Adult
Passage number reprogrammed: P4
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Other:From the same donor: UNEWi026-A, UNEWi026-C
Characterization
Marker expression: SSEA-1(-),NANOG (+),TRA 1-60 (+),SSEA-4 (+)
Differentiation potency:Ectoderm: TUJ-1 (+),Endoderm: Alpha FetoProtein(+),Mesoderm: SMA (+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: P11
Cell line karyotype: No clinically significant imbalance was detected
Karyotyping method: Molecular karyotyping by SNP array
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 20%
Temperature: 37°CLegal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) reprogramming method Sendai virus description age (60-64) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) diabetes (mellitus) shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UNEWi026-C 66540446-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of NewcastleCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Primary cell developmental stage: Adult
Passage number reprogrammed: P4
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Other:From the same donor: UNEWi026-A, UNEWi026-B
Characterization
Marker expression: NANOG (+),TRA 1-60 (+),SSEA-4 (+),SSEA-1 (-)
Differentiation potency:Ectoderm: TUJ-1 (+),Endoderm: Alpha FetoProtein(+),Mesoderm: SMA (+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: P12
Cell line karyotype: No clinically significant imbalance was detected
Karyotyping method: Molecular karyotyping by SNP array
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 20%
Temperature: 37°CLegal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) reprogramming method Sendai virus description age (60-64) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) diabetes (mellitus) shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UNEWi027-A 66540406-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of NewcastleCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Primary cell developmental stage: Adult
Passage number reprogrammed: P4
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown
Characterization
Analysis of Undifferentiated Cells
Marker expression: SSEA-4 (+)TRA 1-60 (+)SSEA-1 (-)NANOG (+)
Differentiation potency
Ectoderm: In vitro spontaneous differentiation
Marker Expressed:TUBB3 (+)
Endoderm: In vitro spontaneous differentiation
Marker Expressed:AFP (+)
Mesoderm: In vitro spontaneous differentiation
Marker Expressed:ACTA2 (+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: P30
Cell line karyotype: No clinically significant imbalance was detected
Karyotyping method: Molecular karyotyping by SNP array
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 20%
Temperature: 37°CLegal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) reprogramming method Sendai virus description age (65-69) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) retinitis pigmentosa shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UOXFi001-A 66540384-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of OxfordCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Tissue collection date: May 3, 2011
Passage number reprogrammed: 4
Reprogramming method
Vector type: Integrating
Vector: Virus
Virus type: Retrovirus
Gene list:
KLF4
MYC
NANOG
POU5F1
SOX2
Absence of reprogramming vector(s): yes
Have the reprogramming vectors been silenced: Unknown
Other:From the same donor: UOXFi001-B, UOXFi001-C, UOXFi001-D
Characterization
Analysis of Undifferentiated Cells
Marker expression: POU5F1 (OCT-4)(+)SSEA-4(+)TRA 1-60 (+)SSEA-1(+)
PluriTest: Pluripotency score: 24.23
Novelty score: 1.40
Microarray data: http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE53425
Report: MK071-1.PNG
Differentiation potency
Ectoderm: In vitro directed differentiation
Marker Expressed:HES5(+)NeuroD1 (+)Pax6(+)
Endoderm: In vitro directed differentiation
Marker Expressed:CXCR4(+)GATA6(+)Sox17(+)
Mesoderm: In vitro directed differentiation
Marker Expressed:MIXL1 (+)NCAM(+)Vimentin(+)
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: -
Genotyping
Karyotyping
Passage number: 11
Cell line karyotype: 46, XX
Karyotyping method: Illumina Human CytoSNP-12v2.1 bead chip arrayLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Matrigel®/Geltrex®
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: mTeSR® 1
Passage Number banked (pre-EBiSC): 8
No. Vials banked (pre-EBiSC): 30Legal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) reprogramming method retrovirus description age (70-74) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) Parkinsons disease shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UOXFi001-B 66540385-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of OxfordCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Tissue collection date: May 3, 2011
Passage number reprogrammed: 4
Reprogramming method
Vector type: Integrating
Vector: Virus
Virus type: Retrovirus
Gene list:
KLF4
MYC
NANOG
POU5F1
SOX2
Absence of reprogramming vector(s): yes
Have the reprogramming vectors been silenced: Unknown
Other:From the same donor: UOXFi001-A, UOXFi001-C, UOXFi001-D
Characterization
Analysis of Undifferentiated Cells
Marker expression: TRA 1-60(+)
PluriTest: Pluripotency score: 23.03
Novelty score: 1.37
Microarray data: http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE53425
Report: UOXFi001-B.PNG
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: -
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: 11
Cell line karyotype: 46, XX
Karyotyping method: Illumina Human CytoSNP-12v2.1 bead chip array
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 20%
Temperature: 37°CLegal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) reprogramming method retrovirus description age (70-74) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) Parkinsons disease shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UOXFi001-C 66540386-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of OxfordCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Tissue collection date: May 3, 2011
Passage number reprogrammed: 4
Reprogramming method
Vector type: Integrating
Vector: Virus
Virus type: Retrovirus
Gene list:
KLF4
MYC
NANOG
POU5F1
SOX2
Absence of reprogramming vector(s): yes
Have the reprogramming vectors been silenced: Unknown
Other:From the same donor: UOXFi001-A, UOXFi001-B, UOXFi001-D
Characterization
Analysis of Undifferentiated Cells
Marker expression: TRA 1-60(+)
PluriTest: Pluripotency score: 23.29
Novelty score: 1.64
Report: UOXFi001-C.PNG
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: -
Genotyping
Karyotyping
Passage number: 13
Cell line karyotype: 46, XX
Karyotyping method: Illumina Human CytoSNP-12v2.1 bead chip arrayLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Matrigel®/Geltrex®
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: mTeSR® 1
Passage Number banked (pre-EBiSC): 9
No. Vials banked (pre-EBiSC): 30Legal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) Quality Level 100, reprogramming method retrovirus description age (70-74) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) Parkinsons disease shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UOXFi001-D 66540387-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of OxfordCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Tissue collection date: May 3, 2011
Passage number reprogrammed: 4
Reprogramming method
Vector type: Integrating
Vector: Virus
Virus type: Retrovirus
Gene list:
KLF4
MYC
NANOG
POU5F1
SOX2
Absence of reprogramming vector(s): yes
Have the reprogramming vectors been silenced: Unknown
Other:From the same donor: UOXFi001-A, UOXFi001-B, UOXFi001-C
Characterization
Analysis of Undifferentiated Cells
Marker expression: TRA 1-60(+)
PluriTest: Pluripotency score: 22.39
Novelty score: 1.44
Report: UOXFi001-D.PNG
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: -
Genotyping
Karyotyping
Passage number: 13
Cell line karyotype: 46, XX
Karyotyping method: Illumina Human CytoSNP-12v2.1 bead chip arrayLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Matrigel®/Geltrex®
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: mTeSR® 1
Passage Number banked (pre-EBiSC): 9
No. Vials banked (pre-EBiSC): 30Legal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) reprogramming method retrovirus description age (70-74) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) Parkinsons disease shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UOXFi002-A 66540388-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of OxfordCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Tissue collection date: May 3, 2011
Passage number reprogrammed: 4
Reprogramming method
Vector type: Integrating
Vector: Virus
Virus type: Retrovirus
Gene list:
KLF4
MYC
NANOG
POU5F1
SOX2
Absence of reprogramming vector(s): yes
Have the reprogramming vectors been silenced: Unknown
Other:From the same donor: UOXFi001-A, UOXFi001-B, UOXFi001-C
Characterization
Analysis of Undifferentiated Cells
Marker expression: TRA 1-60(+)
PluriTest: Pluripotency score: 22.39
Novelty score: 1.44
Report: UOXFi001-D.PNG
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: -
Genotyping
Karyotyping
Passage number: 13
Cell line karyotype: 46, XX
Karyotyping method: Illumina Human CytoSNP-12v2.1 bead chip arrayLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Matrigel®/Geltrex®
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: mTeSR® 1
Passage Number banked (pre-EBiSC): 9
No. Vials banked (pre-EBiSC): 30Legal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) reprogramming method retrovirus description age (50-54) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) Parkinsons disease shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UOXFi002-B 66540389-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of OxfordCell Line DescriptionDerivation
Primary cell type: Fibroblast
Tissue collection date: June 21, 2011
Passage number reprogrammed: 6
Reprogramming method
Vector type: Integrating
Vector: Virus
Virus type: Retrovirus
Gene list:
KLF4
MYC
NANOG
POU5F1
SOX2
Absence of reprogramming vector(s): yes
Have the reprogramming vectors been silenced: Unknown
Other:From the same donor: UOXFi002-A, UOXFi002-C
Characterization
Analysis of Undifferentiated Cells
Marker expression: NANOG(+)TRA 1-60(+)
PluriTest: Pluripotency score: 15.37
Novelty score: 1.46
Report: MK82-30.PNG
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: 12
Cell line karyotype: 46, XY
Karyotyping method: Illumina Human CytoSNP-12v2.1 bead chip array
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 20%
Temperature: 37°CLegal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) Quality Level 100, reprogramming method retrovirus description age (50-54) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) Parkinsons disease shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UOXFi002-C 66540390-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of OxfordCell Line DescriptionDerivation
Primary cell type: -
Reprogramming method
Vector type: None
Other:From the same donor: UOXFi002-A, UOXFi002-B
Characterization
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: -
Genotyping
No Information availableLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineNo Information availableLegal InformationEBiSC is a trademark of Fraunhofer-GesellschaftПараметрыbiological source human dermis (fibroblast) reprogramming method retrovirus description age (50-54) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) Parkinsons disease shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UOXFi003-A 66540391-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of OxfordCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Tissue collection date: July 6, 2011
Passage number reprogrammed: 5
Reprogramming method
Vector type: Integrating
Vector: Virus
Virus type: Retrovirus
Gene list:
KLF4
MYC
NANOG
POU5F1
SOX2
Absence of reprogramming vector(s): yes
Have the reprogramming vectors been silenced: Unknown
Other:From the same donor: UOXFi003-C
Characterization
Analysis of Undifferentiated Cells
Marker expression: TRA 1-60(+)
hPSC Scorecard: Self-renewal,Endoderm,Mesoderm,Ectoderm
PluriTest: Pluripotency score: 23.43
Novelty score: 1.67
Microarray data: http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE53425
Report: UOXFi003-A.PNG
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: -
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: 11
Cell line karyotype: 46, XY
Karyotyping method: Illumina Human CytoSNP-12v2.1 bead chip array
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 20%
Temperature: 37°CLegal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) reprogramming method retrovirus description age (45-49) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) Parkinsons disease shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UOXFi003-C 66540392-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of OxfordCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Tissue collection date: July 6, 2011
Passage number reprogrammed: 4
Reprogramming method
Vector type: Integrating
Vector: Virus
Virus type: Retrovirus
Gene list:
KLF4
MYC
NANOG
POU5F1
SOX2
Absence of reprogramming vector(s): yes
Have the reprogramming vectors been silenced: Unknown
Other:From the same donor: UOXFi003-A
Characterization
Analysis of Undifferentiated Cells
Marker expression: TRA 1-60(+)
PluriTest: Pluripotency score: 36.84
Novelty score: 1.48
Report: UOXFi003-C.PNG
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: -
Genotyping
Karyotyping
Passage number: 12
Cell line karyotype: 46, XY
Karyotyping method: Illumina Human CytoSNP-12v2.1 bead chip arrayLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Matrigel®/Geltrex®
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: mTeSR® 1
Passage Number banked (pre-EBiSC): 8
No. Vials banked (pre-EBiSC): 30Legal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) Quality Level 100, reprogramming method retrovirus description age (45-49) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) Parkinsons disease shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
UOXFi004-A 66540393-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of OxfordCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Tissue collection date: June 1, 2013
Reprogramming method
Vector type: Integrating
Vector: Virus
Virus type: Retrovirus
Gene list:
KLF4
MYC
NANOG
POU5F1
SOX2
Absence of reprogramming vector(s): yes
Have the reprogramming vectors been silenced: Unknown
From the same donor: UOXFi004-B
Characterization
Analysis of Undifferentiated Cells
Marker expression: NANOG(+)TRA 1-60(+)
PluriTest: Pluripotency score: 31.38
Novelty score: 1.36
Report: OX1-18.PNG
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: Negative
Genotyping
Karyotyping
Passage number: 26
Cell line karyotype: 46, XY
Karyotyping method: CytoSNP-12v2.1 bead chip arrayLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Matrigel®/Geltrex®
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: mTeSR® 1
Passage Number banked (pre-EBiSC): 25Legal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) Quality Level 100, reprogramming method retrovirus description age (35-39) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) none shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену
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