Anti-AKR1C3 antibody, Mouse monoclonal

MDL number:	MFCD06798693
NACRES:	NA.41

Кат. номер

A6229-200UL

A6229-25UL

Anti-AKR1C3 antibody, Mouse monoclonal (humanα - hydroxysteroid dehydrogenase, type 2,3; 17β- hydroxysteroid dehydrogenase, type 5) (mouse IgG1 isotype) is derived from the hybridoma NP6.G6. A6 produced by the fusion of mouse myeloma cells (SP20 cells) and splenocytes from BALB/c mice immunized with human AKR1C3 protein. Oxidoreduction of natural and foreign substrates is performed by three enzymes superfamilies, one of them being the AKRs (aldo-keto reductases) family. This family contains 114 proteins that are expressed in prokaryotes and eukaryotes and is divided into 14 subfamilies (AKR1-AKR14). The AKR1 family is the largest among the different fourteen families and contains the aldose reductases, aldehyde reductases, hydroxysteroid dehydrogenases, and steroid 5 β-reductases. AKR1Cs are expressed in many tissues.
human AKR1C3 protein.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper),
Mouse monoclonal clone NP6.G6.A6 anti-AKR1C3 antibody used to tag aldo-keto reductase 1C3 for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques. It is used as a probe to determine the presence and roles of aldo-keto reductase 1C3 in the regulation of the androgen 5-dihydrotestosterone (DHT) and 3-diol compound levels in vivo.
Immunohistochemistry on human placentas were performed using monoclonal mouse anti-AKR1C3 at a dilution of 1:100 or western blot at 1:500.
Anti-AKR1C3 antibody, Mouse monoclonal has been used in

• western blotting
• immunohistochemistry
• enzyme linked immunoassay (ELISA).

Biochem/physiol Actions
In humans AKR1C3 (aldo-keto reductase family 1 member C3) including AKR1C1,2 and 4, catalyze the reduction of the androgen 5-dihydrotestosterone (DHT) into inactive 3 or 3-androstane diol (3/-Diol). AKR1C1-4 isoforms also display 3[17]-hydroxysteroid oxidase activity using 3-Diol as a substrate, in vitro. In non-small cell lung carcinoma, AKR1Cs expression is dramatically increased. Due to their product profile and discrete tissue localization, they may regulate the level of active androgens, estrogens, and progestins in target tissues.
Target description
AKR1C3, aldo-keto reductase family 1, member C3 (3-α hydroxysteroid dehydrogenase, type II), encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	200
biological source	mouse
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	NP6.G6.A6, monoclonal
form	buffered aqueous solution
mol wt	antigen ~38 kDa
species reactivity	human
packaging	antibody small pack of 25 µL
application(s)	immunohistochemistry: suitable","indirect ELISA: suitable","microarray: suitable","western blot: 0.25-0.5 µg/mL using cytosolic fraction extract of A549 human lung carcinoma cell
isotype	IgG1
conjugate	unconjugated
UniProt accession no.	P42330,
shipped in	dry ice
storage temp.	20°C
Gene Information	human ... AKR1C3(8644)

Personal Protective Equipment	Eyeshields,, Gloves,, multi-purpose combination respirator cartridge (US),
RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	Not applicable
Flash Point C	Not applicable