Anti-ALS2 (N-terminal region) antibody produced in rabbit

NACRES:

NA.41

Кат. номер

SAB4200137-25UL

SAB4200137-200UL

Amyotrophic lateral sclerosis (ALS)2 gene codes for 184 kDa alsin protein. Alsin is characterized with the three putative guanine exchange factor (GEF) domains such as, RCC1-like domain (RLD), the Dbl and pleckstrin homology (DH/PH) domain, and a vacuolar protein sorting 9 (VPS9) domain. In addition, ALS2 also contains eight consecutive membrane occupation and recognition nexus (MORN) motifs. ALS2 mRNA is ubiquitously expressed in the CNS and non-neuronal tissues, with the highest expression in the cerebellum and kidney.
Specificity
Anti-ALS2 (N-terminal region) specifically recognizes human, rat, and mouse ALS2.
Anti-ALS2 (N-terminal region) antibody produced in rabbit has been used in immunoblotting and immunoprecipitation.
Biochem/physiol Actions
Amyotrophic lateral sclerosis (ALS)2 has been shown to mediate the activation of Rab5 and Rac1/PACK1. In the nervous system, ALS2 is preferentially associated with the cytoplasmic side of endosome membrane, it modulates endosome membrane trafficking and promotes neurite growth in neuronal cultures. Loss-of function mutations in the ALS2 gene leads to the development of recessive motor neuron diseases, including forms of ALS (ALS2), juvenile PLS (PLSJ) and hereditary spastic paraplegia (HSP).
Physical form
Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
antibody form	affinity isolated antibody
antibody product type	primary antibodies
clone	polyclonal
form	buffered aqueous solution
mol wt	antigen ~185 kDa
packaging	antibody small pack of 25 µL
concentration	~1.5 mg/mL
application(s)	indirect immunofluorescence: 2-4 µg/mL using NIH3T3 cells, western blot: 1.5-3.0 µg/mL using HEK-293T cell lysate over expressing human ALS2, and rat cerebellum extract (S1 fraction).
conjugate	unconjugated
shipped in	dry ice
storage temp.	20°C
Gene Information	mouse ... Als2(74018) rat ... Als2(363235)

RIDADR	NONH for all modes of transport
Flash Point F	Not applicable
Flash Point C	Not applicable