Anti-APC11 Antibody, clone CRQ1.1

eCl@ss:

32160702

Кат. номер

MABE1789-25UG

MABE1789-100UG

Anaphase-promoting complex subunit 11 (UniProt: Q9NYG5; also known as APC11, Cyclosome subunit 11, Hepatocellular carcinoma-associated RING finger protein) is encoded by the ANAPC11 (also known as HSPC214) gene (Gene ID: 51529) in human. APC11 is one of the components of the anaphase-promoting complex (APC) that belongs to the RING-box family of proteins. APC is a large E3 ubiquitin ligase that responsible for targeting proteins for degradation during mitosis. The APC is assembled from 13 different proteins where in APC2 binds to APC11 forming the catalytic core that recruits the E2 ubiquitin conjugating enzyme. APC11 has with multiple zinc -binding sites with a RING-type zinc-finger domain (aa 34-77). It is expressed at high levels in the skeletal muscle and heart and in moderate levels in brain, kidney, and liver. Two isoforms of APC11 have been described that are produced by alternative splicing. (Ref.: Schreiber, A et al., (2011). Nature. 470(7333); 227-32).
Specificity
Clone CRQ1.1 specifically detects human Anaphase-promoting complex subunit 11 (APC11). It targets an epitope with in 9 amino acids from the C-terminal region.
Epitope: C-terminus
A linear peptide corresponding to 9 amino acids from the C-terminal region of human Anaphase-promoting complex subunit 11 (APC11).
Anti-APC11, clone CRQ1.1, Cat. No. MABE1789, is a highly specific mouse monoclonal antibody that targets Anaphase-promoting complex subunit 11 and has been tested in Western Blotting.
Western Blotting Analysis: A representative lot detected APC11 in Western Blotting applications (Sedgwick, G.G., et. al. (2013). EMBO J. 32(2):303-14).
Western Blotting Analysis: A representative lot detected APC11 in APC11-Flag expressed in HeLa cells following tetracycline treatment.
Research Category
Epigenetics & Nuclear Function
Quality
Isotype testing: Identity Confirmation by Isotyping Test.
Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG2a.
Target description
9.84 kDa calculated.
Physical form
Format: Purified
Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Protein G purified
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	mouse
antibody form	purified antibody
antibody product type	primary antibodies
clone	CRQ1.1, monoclonal
species reactivity	human
species reactivity (predicted by homology)	bovine (based on 100% sequence homology), monkey (based on 100% sequence homology), rat (based on 100% sequence homology)
packaging	antibody small pack of 25 µg
application(s)	western blot: suitable
isotype	IgG2a
NCBI accession no.	NP_001002244.1,
UniProt accession no.	Q9NYG5,
Gene Information	human ... ANAPC11(51529)