Anti-Apolipoprotein A-I Antibody, clone Wt20-7

eCl@ss:

32160702

Кат. номер

MABS1310-25UL

MABS1310-100UL

Apolipoprotein A-I (UniProt: P02647; also known as Apo-AI, ApoA-I, Apolipoprotein A1) is encoded by the APOA1 gene (Gene ID: 335) in human. Apo A-1 is a major protein of plasma high density lipoprotein (HDL) and is also found in chylomicrons. It plays a major role in transporting cholesterol from peripheral tissues to the liver. It is synthesized mainly in the liver with a signal peptide (aa 1-18), which is proteolytically cleaved to generate the mature protein. Apo A-1 also serves as a co-factor for lecithin cholesterolacyltransferase (LCAT) that is involved in the formation of cholesteryl esters. Elevated levels of oxidized Apo A-1 (on Methionine 110 and 136) have been linked to increased risk for coronary artery disease. Mutations in APOA1 gene have also been linked to HDL deficiencies that lead to premature coronary artery disease and a reduction in cellular cholesterol efflux. Mutations in APOA1 gene have also shown to result in amyloidosis with extensive visceral amyloid deposit and in the pathogenesis of amyloid polyneuropathy-nephropathy Iowa type, also known as familial amyloid polyneuropathy type III.
Specificity
Clone Wt20-7 specifically detects human Apolipoprotein A-I.
GST-tagged full-length human recombinant Apolipoprotein A-I
Anti-Apolipoprotein A-I, clone Wt20-7, Cat. No. MABS1310, is a mouse monoclonal antibody that detects human Apolipoprotein A-I and has been tested for use in Dot Blot, ELISA, Immunocytochemistry, and Western Blotting.
Research Category
Signaling
ELISA Analysis: A representative lot detected Apolipoprotein A-I in ELISA applications (Kimura, H., et. al. (2017). Sci Rep. 7(1):2988).
Western Blotting Analysis: A representative lot detected Apolipoprotein A-I in Western Blotting applications (Kuwabara, K., et. al. (2015). J Biol Chem. 290(40):24210-21).
Immunocytochemistry Analysis: A representative lot detected Apolipoprotein A-I in Immunocytochemistry applications (Kameyama, H., et. al. (2016). Sci Rep. 6:30391; Kuwabara, K., et. al. (2015). J Biol Chem. 290(40):24210-21).
Dot Blot Analysis: A representative lot detected Apolipoprotein A-I in Dot Blot applications (Kimura, H., et. al. (2017). Sci Rep. 7(1):2988; Kameyama, H., et. al. (2016). Sci Rep. 6:30391; Kuwabara, K., et. al. (2015). J Biol Chem. 290(40):24210-21).
Quality
Evaluated by Western Blotting in human fetal liver tissue lysate.
Western Blotting Analysis: A 1:2,000 dilution of this antibody detected Apolipoprotein A-I in human fetal liver tissue lysate.
Target description
~28 kDa observed; 30.78 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Format: Purified
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Protein G purified
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	mouse
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	Wt20-7, monoclonal
species reactivity	human
packaging	antibody small pack of 25 µL
application(s)	ELISA: suitable","dot blot: suitable","immunocytochemistry: suitable","western blot: suitable
isotype	IgG1
NCBI accession no.	NP_000030,
UniProt accession no.	P02647,

WGK Germany	WGK 2
Flash Point F	Not applicable
Flash Point C	Not applicable