Anti-MLL-3

eCl@ss:	32160702
NACRES:	NA.41

Кат. номер

ABE1851

ABE1851-25UG

Histone-lysine N-methyltransferase 2D (EC 2.1.1.43; UniProt O14686; also known as MLL4, ALL1-related protein, Lysine N-methyltransferase 2D) is encoded by the KMT2D (also known as MLL4, ALR, KABUK1, MLL2) gene (Gene ID: 8085) in human. MLL4 methylates Lys-4 of histone H3 to form H3K4 mono- and di-methylation (H3K4me1/2), which represents a specific tag for transcriptional enhancers. MLL4 is also a component of the MLL3/4 protein complex that plays an important role during cell differentiation and animal development. MLL4 is localized to the nucleus and is expressed in most embryonic and adult tissues. Defects in MLL4 (often known as MLL2 and KMT2D in the literature) have been linked to Kabuki syndrome and congenital heart diseases. Inactivating mutations in MLL4/MLL2/KMT2D gene have been linked to many types of cancers, including medulloblastoma, non-Hodgkin lymphoma, diffuse large B-cell lymphoma, prostate cancer, breast cancer, hepatocellular carcinoma, ovarian cancer, lung, bladder, head & neck cancers, and pancreatic cancer. (Ref.: Lee, J.E., et al. (2013). Elife. 2:e01503; Lawrence, M.S., et al., (2014). Nature. 505(7484):495-501).
Specificity
This polyclonal antiody detects MLL-3 protein in human, mouse and rat cells. It targets and epitope within 18 amino acids from the internal region of N-terminal half.
A linear peptide corresponding to 18 amino acids from the N-terminal region of human MLL-3.
Epitope: unknown
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected MLL-3 in mouse testis, rat uterus, and mouse kidney tissues.
Chromatin Immunoprecipitation Analysis: A representative lot detected MLL-3 in MEF cells (Lee, J., et. al. (2009) Proc Natl Acad Sci USA. 106(21):8513-8).
Western Blotting Analysis: A representative lot detected MLL-3 in Western Blotting applications (Lee, J., et. al. (2009) Proc Natl Acad Sci USA. 106(21):8513-8); Bres, V., et. al. (2009). Mol Cell. 36(1):75-87).
Immunohistochemistry Analysis: A representative lot detected MLL-3 in mouse urothelial tumors (Lee, J., et. al. (2009) Proc Natl Acad Sci USA. 106(21):8513-8).
Anti-MLL3, Cat. No. ABE1851, is a highly specific rabbit polyclonal antibody that targets Histone-lysine N-methyltransferase 2C and has been tested in Chromatin Immunoprecipitation (ChIP), Immunohistochemistry (Paraffin), and Western Blotting.
Research Category
Epigenetics & Nuclear Function
Quality
Evaluated by Western Blotting in U2OS nuclear extract.
Western Blotting Analysis: 1 µg/mL of this antibody detected MLL-3 in 10 µg of U2OS cell nuclear extract.
Target description
~540 kDa observed; 540.19 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Affinity Purified
Format: Purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	100
antibody form	affinity isolated antibody
antibody product type	primary antibodies
clone	polyclonal
species reactivity	rat, mouse, human
packaging	antibody small pack of 25 µg
application(s)	ChIP: suitable","immunohistochemistry: suitable (paraffin)","western blot: suitable
isotype	IgG
NCBI accession no.	NP_001074852,
UniProt accession no.	Q8BRH4,
shipped in	ambient

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable