Anti-Neuritin (CPG15) Antibody, clone A6.1

eCl@ss:

32160702

Кат. номер

MABN1840-25UG

MABN1840-100UG

Neuritin (UniProt: Q9NPD7) is encoded by the NRN1 (also known as NRN) gene (Gene ID: 51299) in human. Neuritin, a conserved, glycosylphosphatidylinositol (GPI)-anchored protein that plays a role in the development, survival and function of neurons. Its activity is induced by brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3). It is highly expressed by induced and natural regulatory T cells (TREGs). It also promotes the maintenance of the TREGs pool by stabilizing Foxp3 expression and promoting the persistence of these cells. Deficiency of Neuritin is shown to result in an imbalance between central and peripheral TREG populations and functions. Neuritin has a wide variety of effects in the nervous system, such as promoting neurite outgrowth, modulating neurite outgrowth during neuronal differentiation, and protecting motor neuron axons. It can also promote dendritic growth, regulate synaptic plasticity, and stabilize active synapses, and promote synaptic maturation. Its expression in the hippocampus is shown to be induced by neuronal activity following chemical- or electrical-induced seizures, ischemia, and exercise. Chronic antidepressant treatment is reported to increase neuritin expression in rat brain. Neuritin is synthesized with a signal peptide (aa 1-27) and a propeptide (aa 28-116) that are cleaved off in the mature form (aa 117-142). (Ref.: Son, H., et al. (2012). Proc. Natl. Acad. Sci. USA. 109(28); 11378-83; Zhou, S., and Zhou, J (2014). Curr. Med. Chem. 21(10); 1212-19). Note: A6.1 hybridoma clone, and WT/KO validation, courtesy of Lauren Makuch and Dr. Elly Nedivi, Massachusetts Institute of Technology.
Specificity
Clone A6.1 detects human and rat Neuritin.
GST-tagged full-length human recombinant Neuritin.
Research Category
Neuroscience
Anti-Neuritin (CPG15), clone A6.1, Cat. No. MABN1840, is a highly specific mouse monoclonal antibody that targets Neuritin and has been tested for use in Western Blotting.
Quality
Evaluated by Western Blotting with recombinant human/rat neuritin.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected recombinant human/rat Neuritin (CPG15).
Target description
~15 kDa observed; 15.33 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G purified
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	mouse
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	A6.1, monoclonal
species reactivity	rat, human
species reactivity (predicted by homology)	monkey (based on 100% sequence homology), bovine (based on 100% sequence homology)
packaging	antibody small pack of 25 µg
application(s)	western blot: suitable
isotype	IgG2b
NCBI accession no.	NP_001265639,
UniProt accession no.	Q9NPD7,
Gene Information	human ... NRN1(51299)

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable