Anti-p190-B RhoGAP (C-terminal region) antibody produced in rabbit

NACRES:

NA.41

Кат. номер

P0045-25UL

P0045-200UL

Rho GTPase activating protein 5 (ARHGAP5) or p190-B RhoGAP is a member of the RhoGTPase activating protein (RhoGAP family). ARHGAP5 gene is mapped to human chromosome 14q12 and is protumorigenic. p190-A and p190-B are the two isoforms that are highly expressed in the embryonic and adult brain.
Specificity
Anti-p190-B RhoGAP (C-terminal region) specifically recognizes rat and mouse p190-B RhoGAP.
Anti-p190-B RhoGAP (C-terminal region) antibody produced in rabbit may be used in immunoblotting.
Biochem/physiol Actions
Rho GTPase activating protein 5 (ARHGAP5) or p190-B RhoGAP functions as negative regulator of Rho activity. Deletion of p190-B in mice results in axonal-tract deficits and neuronal differentiation defects, indicating a central role of p190-B in normal brain development. Also, p190-B-deficient mice are reduced in size due to impaired insulin and insulin-like growth factors (IGF) signaling that affect adipogenesis. Several studies indicate a critical interaction between p190-B and IGF signaling pathway during embryonic mammary morphogenesis and epithelial progenitor cell migration. p190-B has shown to regulate the expression of membrane-type 1-matrix metalloproteinase (MT1-MMP) and matrix metalloproteinase 2 (MMP2) in endothelial cells and in modulating matrix remodeling and angiogenesis processes. It displays a elevated expression in hepatocellular cancer and promotes tumor spreading.
Physical form
Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	200
antibody form	affinity isolated antibody
antibody product type	primary antibodies
clone	polyclonal
form	buffered aqueous solution
mol wt	antigen ~190 kDa
species reactivity	mouse, rat
packaging	antibody small pack of 25 µL
concentration	~1.5 mg/mL
application(s)	western blot: 2-4 µg/mL using rat brain extract (S1 fraction) and a C2C12 lysate
conjugate	unconjugated
UniProt accession no.	P97393,
shipped in	dry ice
storage temp.	20°C
Gene Information	human ... ARHGAP5(394) mouse ... Arhgap5(11855) rat ... Arhgap5(299012)

WGK Germany

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