Stem Cells
- Сортировать:
- Вид таблицей
-
CENSOi003-B 66540600-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Censo BiotechnologiesCell Line DescriptionDerivation
Primary cell type: Fibroblast
Tissue collection year: 2012
Passage number reprogrammed: 6
Reprogramming method
Vector type: None
Vector Free Reprogramming
Types of factor(s) used: mRNA
mRNA: NANOG
LIN28
MYC
KLF4
SOX2
POU5F1
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
SSEA-4 Yes
POU5F1 (OCT-4) Yes
SSEA-1 No
TRA 1-60 Yes
Differentiation potency
Ectoderm:
Ectoderm
In vitro spontaneous differentiation
Marker Expressed
HES5 Yes
NeuroD1 Yes
PAX6 Yes
Endoderm:
Endoderm
In vitro spontaneous differentiation
Marker Expressed
CXCR4 Yes
GSC Yes
PITX1 Yes
Mesoderm:
Mesoderm
In vitro spontaneous differentiation
Marker Expressed
NCAM1 Yes
VIM Yes
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: 11
Cell line karyotype: No autosomal or sex chromosome aneuploidies were detected
Karyotyping method: KaryoLite BoBs
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 21%
Temperature: 37°CLegal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) reprogramming method mRNA description age (N/A) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) muscular dystrophy (Duchenne) shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
CENSOi005-A 66540601-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Censo BiotechnologiesCell Line DescriptionDerivation
Primary cell type: Fibroblast
Tissue collection year: 2014
Passage number reprogrammed: 5
Reprogramming method
Vector type: None
Vector Free Reprogramming
Types of factor(s) used: mRNA
mRNA: NANOG
LIN28
MYC
KLF4
SOX2
POU5F1
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
SSEA-1 No
SSEA-4 Yes
TRA 1-60 Yes
POU5F1 (OCT-4) Yes
Differentiation potency
Ectoderm:
Ectoderm
In vitro spontaneous differentiation
In vitro directed differentiation
Marker Expressed
HES5 Yes
NeuroD1 Yes
PAX6 Yes
Endoderm:
Endoderm
In vitro directed differentiation
Marker Expressed
CXCR4 Yes
GATA6 Yes
SOX17 Yes
Mesoderm:
Mesoderm
In vitro spontaneous differentiation
In vitro directed differentiation
Marker Expressed
MIXL1 Yes
NCAM1 Yes
VIM Yes
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: 12
Cell line karyotype: 46,XY
Karyotyping method: G-Banding
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 21%
Temperature: 37°CLegal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) reprogramming method mRNA description age (5-9) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) muscular dystrophy (Duchenne) shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
CENSOi007-A 66540602-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Censo BiotechnologiesCell Line DescriptionDerivation
Primary cell type: Fibroblast
Tissue collection year: 2014
Passage number reprogrammed: 5
Reprogramming method
Vector type: None
Vector Free Reprogramming
Types of factor(s) used: mRNA
mRNA: LIN28
NANOG
MYC
KLF4
SOX2
POU5F1
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4) Yes
SSEA-1 No
TRA 1-60 Yes
SSEA-4 Yes
Differentiation potency
Ectoderm:
Ectoderm
In vitro spontaneous differentiation
Marker Expressed
HES5 Yes
NeuroD1 Yes
PAX6 Yes
Endoderm:
Endoderm
In vitro spontaneous differentiation
Marker Expressed
CXCR4 Yes
GATA6 Yes
Mesoderm:
Mesoderm
In vitro spontaneous differentiation
Marker Expressed
NCAM1 Yes
VIM Yes
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: 11
Cell line karyotype: No autosomal or sex chromosome aneuploidies were detected
Karyotyping method: KaryoLite BoBs
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 21%
Temperature: 37°CLegal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) reprogramming method mRNA description age (10-14) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) muscular dystrophy (Duchenne) shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
CENSOi008-A 66540603-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Censo BiotechnologiesCell Line DescriptionDerivation
Primary cell type: Fibroblast
Tissue collection year: 2015
Reprogramming method
Vector type: None
Vector Free Reprogramming
Types of factor(s) used: mRNA
mRNA: NANOG
LIN28
MYC
KLF4
SOX2
POU5F1
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
SSEA-1 No
POU5F1 (OCT-4) Yes
TRA 1-60 Yes
SSEA-4 Yes
Differentiation potency
Ectoderm:
Ectoderm
In vitro spontaneous differentiation
Marker Expressed
HES5 Yes
NeuroD1 Yes
PAX6 Yes
Endoderm:
Endoderm
In vitro spontaneous differentiation
Marker Expressed
GATA6 Yes
MIXL1 Yes
Mesoderm:
Mesoderm
In vitro spontaneous differentiation
Marker Expressed
NCAM1 Yes
VIM Yes
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: 10
Cell line karyotype: No autosomal or sex aneuploidies were detected in this sample
Karyotyping method: KaryoLite BoBs
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 21%
Temperature: 37°CLegal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) reprogramming method mRNA description age (25-29) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) muscular dystrophy (myotonic type 1) shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
EDi001-A 66540058-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of EdinburghCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Primary cell developmental stage: Adult
Reprogramming method
Vector type: Integrating
Vector: Virus
Virus type: Retrovirus
Have the reprogramming vectors been silenced: Unknown
Characterization
Analysis of Undifferentiated Cells
Marker expression: TRA 1-60(+)POU5F1 (OCT-4)(+)SSEA-1(-)SSEA-4 (+)
Differentiation potency
Ectoderm: In vitro spontaneous differentiation
Marker Expressed:HES5(+)NeuroD1(+)PAX6(+)
Endoderm: In vitro spontaneous differentiation
Marker Expressed:CXCR4(+)GATA6(+)SOX17(+)
Mesoderm: In vitro spontaneous differentiation
Marker Expressed:NCAM1 (+)PECAM1(+)VIM(+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: P23
Cell line karyotype: 46,XX
Karyotyping method: G-Banding
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA)
- Cell Line Information Pack (CLIP)
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 21%
Temperature: 37°CLegal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) reprogramming method retrovirus description age (50-54) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) Parkinsons disease shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point(F) Not applicable Flash Point(C) Not applicable Узнать цену -
EDi001-A-1 66540166-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of EdinburghCell Line DescriptionDerivation
Primary cell type: -
Reprogramming method
Vector type: Integrating
Vector: Virus
Virus type: Retrovirus
Gene list:
KLF4
MYC
POU5F1
SOX2
Have the reprogramming vectors been silenced: Yes
Merthods used: immune_staining, rtpcr
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4) Yes
SSEA-1 No
SSEA-4 Yes
TRA 1-60 Yes
Differentiation potency
Ectoderm:
Ectoderm
In vitro spontaneous differentiation
Endoderm:
Endoderm
In vitro spontaneous differentiation
Mesoderm:
Mesoderm
In vitro spontaneous differentiation
Microbiology / Virus Screening
HIV 1: Not done
HIV 2: Not done
Hepatitis B: Not done
Hepatitis C: Not done
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.
Genetic Modification
Disease/phenotype related modifications
Disease: Parkinsons disease
Type of modification: Isogenic
Gene: SNCA
Chromosome location: 4q22.1
Target locus modification: This clone was picked in parallel to other Nickase pair targeted clones (EDi001-A2/A3 and A4) but no SNCA alleles were lost in this clone. Therefore all 4 alleles remain, and it retains the donor heterozygous triplication of SCNA.
Description: CRISPR/Cas was employed to create a mutation in Exon2, disrupting the ATG start site of SCNA. The site targeted for editing was sequenced for 8 clones; however, in these 8 clones, none of the sites had been modified. Therefore, it appears that the cell line had not been edited. The clone was not further examined for off-target modifications.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 20%
Temperature: 37°CLegal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human reprogramming method retrovirus description age (N/A) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) Parkinsons disease shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
EDi001-A-2 66540167-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of EdinburghCell Line DescriptionDerivation
Primary cell type: -
Reprogramming method
Vector type: Integrating
Vector: Virus
Virus type: Retrovirus
Gene list:
KLF4
MYC
POU5F1
SOX2
Have the reprogramming vectors been silenced: Yes
Merthods used: rtpcr
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Microbiology / Virus Screening
HIV 1: Not done
HIV 2: Not done
Hepatitis B: Not done
Hepatitis C: Not done
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.
Genetic Modification
Disease/phenotype related modifications
Disease: Parkinsons disease
Details: This is a PD line, with the control being NAS lines and CRISPR/Cas9-corrected AST22 lines
Type of modification: Gene knock-out
Gene: SNCA
Chromosome location: 4q22.1
Delivery method: CRISPR-associated (CRISPR/Cas) System
Description: CRISPR/Cas was employed to create a mutation in Exon2, disrupting the ATG start site and some sequence 5 to the coding start of SCNA. PCR amplicons of the CRISPR site were cloned using the TOPO cloning technique. Sequencing of 8 TOPO clones detected 2 deletions and 1 insertion; however, sequencing of the CRISPR sites shows that only one of these modifications disrupts the ATG site. This indicates 1 allele was putatively knocked out, and 3 alleles remain. Additional comment: there are two additional ATG sites located immediately downstream of the normal ATG start site, one of which is in-frame (9 bases) to the initial ATG. It is possible that inactivation of the first ATG start site might not be completely sufficient to prevent translation from a second downstream ATG.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 20%
Temperature: 37°CLegal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human reprogramming method retrovirus description age (N/A) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) Parkinsons disease shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
EDi001-A-3 66540168-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of EdinburghCell Line DescriptionDerivation
Primary cell type: -
Reprogramming method
Vector type: Integrating
Vector: Virus
Virus type: Retrovirus
Gene list:
KLF4
MYC
POU5F1
SOX2
Have the reprogramming vectors been silenced: Yes
Merthods used: rtpcr
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Microbiology / Virus Screening
HIV 1: Not done
HIV 2: Not done
Hepatitis B: Not done
Hepatitis C: Not done
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.
Genetic Modification
Disease/phenotype related modifications
Disease: Parkinsons disease
Type of modification: Gene knock-out
Gene: SNCA
Chromosome location: 4q22.1
Delivery method: CRISPR-associated (CRISPR/Cas) System
Description: CRISPR/Cas was employed to create a mutation in Exon2, disrupting the ATG start site and some sequence 5 to the coding start of SCNA. PCR amplicons of the CRISPR site were cloned using the TOPO cloning technique. Sequencing of 8 TOPO clones detected 3 deletions; however, sequencing of the CRISPR sites shows that only one of these modifications disrupts the ATG site. This indicates 1 allele was putatively knocked out, and 3 alleles remain. Additional comment: there are two additional ATG sites located immediately downstream of the normal ATG start site, one of which is in-frame (9 bases) to the initial ATG. It is possible that inactivation of the first ATG start site might not be completely sufficient to prevent translation from the second downstream ATG, which is in-frame to the first.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 20%
Temperature: 37°CLegal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human reprogramming method retrovirus description age (N/A) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) Parkinsons disease shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
EDi002-A 66540059-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of EdinburghCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Primary cell developmental stage: Adult
Reprogramming method
Vector type: Integrating
Vector: Virus
Virus type: Retrovirus
Have the reprogramming vectors been silenced: Unknown
Characterization
Analysis of Undifferentiated Cells
Marker expression: SSEA-4(+)POU5F1 (OCT-4)(+)SSEA-1 (-) TRA 1-60 (+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: 48
Cell line karyotype: 46,XX
Karyotyping method: G-Banding
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 21%
Temperature: 37°CLegal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) Quality Level 100, reprogramming method retrovirus description age (30-34) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) none shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
EDi008-B 66540061-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of EdinburghCell Line DescriptionDerivation
Primary cell type: Fibroblast
Reprogramming method
Vector type: Non-integrating
Vector: Episomal
Gene list:
KLF4
Lin28
L-myc
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
SSEA-1 No
POU5F1 (OCT-4) Yes
SSEA-4 Yes
Differentiation potency
Ectoderm:
Ectoderm
In vitro spontaneous differentiation
Endoderm:
Endoderm
In vitro spontaneous differentiation
Mesoderm:
Mesoderm
In vitro spontaneous differentiation
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: 11
Cell line karyotype: 46,XX,der(15)t(1;15)(q12;p11.2)[9]/46,XX[2]
Karyotyping method: G-Banding
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: Essential E8®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 21%
Temperature: 37°CLegal InformationE8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation
EBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.Параметрыbiological source human dermis (fibroblast) reprogramming method episomal description age (N/A) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) Parkinsons disease shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
EDi010-A 66540447-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of EdinburghCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown
Other:From the same donor: EDi010-B
Characterization
Analysis of Undifferentiated Cells
Marker expression: SSEA-4 (+)TRA 1-60(+)POU5F1 (OCT-4)(+)SSEA-1(-)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Not done
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: -
Genotyping
Passage number: 21
Cell line karyotype: No autosomal or sex chromosome aneuploidies detected
Karyotyping method: Karyolite BoBsLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Vitronectin
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: Essential E8®Legal InformationE8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation
EBiSC is a trademark of Fraunhofer-GesellschaftПараметрыbiological source human dermis (fibroblast) reprogramming method Sendai virus description age (N/A) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) bipolar disorder shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
EDi010-B 66540448-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of EdinburghCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown
Characterization
Analysis of Undifferentiated Cells
Marker expression: POU5F1 (OCT-4)(+)SSEA-4(+)TRA 1-60(+)SSEA-1(-)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Not done
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: -
Genotyping
Karyotyping
Passage number: 16
Cell line karyotype: No autosomal or sex chromosome aneuploidies detected
Karyotyping method: Karyolite BoBsLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Vitronectin
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: Essential E8®Legal InformationE8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation
EBiSC is a trademark of Fraunhofer-GesellschaftПараметрыbiological source human dermis (fibroblast) reprogramming method Sendai virus description age (N/A) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) bipolar disorder shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
EDi011-A 66540449-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of EdinburghCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown
Other:From the same donor: EDi011-B, EDi011-C
Characterization
Analysis of Undifferentiated Cells
Marker expression: TRA 1-60(+)SSEA-4(+)POU5F1 (OCT-4)(+)SSEA-1(-)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Not done
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: -
Genotyping
Karyotyping
Passage number: 23
Cell line karyotype: No autosomal or sex chromosome aneuploidies detected
Karyotyping method: Karyolite BoBsLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Vitronectin
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: Essential E8®Legal InformationE8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation
EBiSC is a trademark of Fraunhofer-GesellschaftПараметрыbiological source human dermis (fibroblast) reprogramming method Sendai virus description age (N/A) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) bipolar disorder shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
EDi011-B 66540450-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of EdinburghCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown
Other:From the same donor: EDi011-A, EDi011-C
Characterization
Analysis of Undifferentiated Cells
Marker expression: TRA 1-60(+)SSEA-1(-)SSEA-4 (+)POU5F1 (OCT-4)(+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Not done
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: -
Genotyping
Karyotyping
Passage number: 26
Cell line karyotype: No autosomal or sex chromosome aneuploidies detected
Karyotyping method: Karyolite BoBsLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Vitronectin
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: Essential E8®Legal InformationE8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation
EBiSC is a trademark of Fraunhofer-GesellschaftПараметрыbiological source human dermis (fibroblast) reprogramming method Sendai virus description age (N/A) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) bipolar disorder shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
EDi011-C 66540451-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of EdinburghCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown
Other:From the same donor: EDi011-A, EDi011-B
Characterization
Analysis of Undifferentiated Cells
Marker expression: POU5F1 (OCT-4) (+)TRA 1-60 (+)SSEA-1 (+)SSEA-4 (+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Not done
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: -
Genotyping
Karyotyping
Passage number: 26
Cell line karyotype: No autosomal or sex chromosome aneuploidies detected
Karyotyping method: Karyolite BoBsLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Vitronectin
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: Essential E8®Legal InformationE8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation
EBiSC is a trademark of Fraunhofer-GesellschaftПараметрыbiological source human dermis (fibroblast) reprogramming method Sendai virus description age (N/A) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) bipolar disorder shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
EDi012-A 66540452-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of EdinburghCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown
Other:From the same donor: EDi012-B, EDi012-C
Characterization
Analysis of Undifferentiated Cells
Marker expression: TRA 1-60(+)POU5F1 (OCT-4)(+)SSEA-4(+) SSEA-1(-)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Not done
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: -
Genotyping
Karyotyping
Passage number: 23
Cell line karyotype: No autosomal or sex chromosome aneuploidies detected
Karyotyping method: BACs-on-BeadsLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Vitronectin
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: Essential E8®Legal InformationE8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation
EBiSC is a trademark of Fraunhofer-GesellschaftПараметрыbiological source human dermis (fibroblast) Quality Level 100, reprogramming method Sendai virus description age (N/A) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) none shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
EDi012-B 66540453-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of EdinburghCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown
Other:From the same donor: EDi012-A, EDi012-C
Characterization
Analysis of Undifferentiated Cells
Marker expression: POU5F1 (OCT-4)(+)SSEA-4 (+)TRA 1-60 (+) SSEA-1 (-)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Not done
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: -
Genotyping
Karyotyping
Passage number: 28
Cell line karyotype: No autosomal or sex chromosome aneuploidies detected
Karyotyping method: BACs-on-BeadsLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Matrigel®/Geltrex®
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: Essential E8®Legal InformationE8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation
EBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.Параметрыbiological source human dermis (fibroblast) reprogramming method Sendai virus description age (N/A) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) none shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
EDi012-C 66540454-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of EdinburghCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown
Other:From the same donor: EDi012-A, EDi012-B
Characterization
Analysis of Undifferentiated Cells
Marker expression: TRA 1-60(+) SSEA-4(+)SSEA-1(-)POU5F1 (OCT-4)(+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Not done
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: -
Genotyping
Karyotyping
Passage number: 22
Cell line karyotype: No autosomal or sex chromosome aneuploidies detected
Karyotyping method: BACs-on-BeadsLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Matrigel®/Geltrex®
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: Essential E8®Legal InformationE8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation
EBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.Параметрыbiological source human dermis (fibroblast) reprogramming method Sendai virus description age (N/A) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) none shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
EDi013-A 66540455-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of EdinburghCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown
Other:From the same donor: EDi013-B, EDi013-C
Characterization
Analysis of Undifferentiated Cells
Marker expression: SSEA-4 (+)SSEA-1 (-)POU5F1 (OCT-4) (+)TRA 1-60 (+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Not done
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: -
Genotyping
Karyotyping
Passage number: 16
Cell line karyotype: No autosomal or sex chromosome aneuploidies were detected
Karyotyping method: BACs-on-BeadsLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Vitronectin
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: Essential E8®Legal InformationE8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation
EBiSC is a trademark of Fraunhofer-GesellschaftПараметрыbiological source human dermis (fibroblast) reprogramming method Sendai virus description age (60-64) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) none shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
EDi013-B 66540456-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of EdinburghCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown
Other:From the same donor: EDi013-A, EDi013-C
Characterization
Analysis of Undifferentiated Cells
Marker expression: SSEA-4 (+)POU5F1 (OCT-4) (+)SSEA-1 (-)TRA 1-60 (+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Not done
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: -
Genotyping
Karyotyping
Passage number: 18
Cell line karyotype: The probes near the telomere on the q arm of chromosome 19 indicate an increased dosage. For all other chromosomes a normal dosage pattern was observed for both the short and the long-arm probes
Karyotyping method: BACs-on-BeadsLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Vitronectin
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: Essential E8®Legal InformationE8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation
EBiSC is a trademark of Fraunhofer-GesellschaftПараметрыbiological source human dermis (fibroblast) reprogramming method Sendai virus description age (60-64) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) none shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
EDi013-C 66540457-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of EdinburghCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Is the reprogramming vector detectable: Unknown
Other:From the same donor: EDi013-A, EDi013-B
Characterization
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Not done
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: -
Genotyping
Karyotyping
Passage number: 23
Cell line karyotype: No autosomal or sex aneuploidies were detected
Karyotyping method: BACs-on-BeadsLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Matrigel®/Geltrex®
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: Essential E8®Legal InformationE8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation
EBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.Параметрыbiological source human dermis (fibroblast) reprogramming method Sendai virus description age (60-64) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) none shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
EDi014-A 66540458-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of EdinburghCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown
Other:From the same donor: EDi014-B
Characterization
Analysis of Undifferentiated Cells
Marker expression: SSEA-4(+),TRA 1-60(+),POU5F1 (OCT-4)(+)
Differentiation potency
Ectoderm:
Endoderm:
Mesoderm:
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Genotyping
Karyotyping
Passage number: 18
Cell line karyotype: No autosomal or sex chromosome aneuploidies were detected
Karyotyping method: Bacs-on-BeadsLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Matrigel®/Geltrex®
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: Essential E8®Legal InformationE8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation
EBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.Параметрыbiological source human dermis (fibroblast) reprogramming method Sendai virus description age (N/A) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) depression (unipolar) shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
EDi014-B 66540459-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of EdinburghCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown
Other:From the same donor: EDi014-A
Characterization
Analysis of Undifferentiated Cells
Marker expression: POU5F1 (OCT-4)(+)SSEA-1 (-)SSEA-4(+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Genotyping
Karyotyping
Passage number: 21
Cell line karyotype: No autosomal or sex chromosome aneuploidies were detected
Karyotyping method: Bacs-on-BeadsLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Matrigel®/Geltrex®
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: Essential E8®Legal InformationE8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation
EBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.Параметрыbiological source human dermis (fibroblast) reprogramming method Sendai virus description age (N/A) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) depression (unipolar) shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
EDi015-A 66540460-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of EdinburghCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown
Other: From the same donor: EDi015-B, EDi015-C
Characterization
Analysis of Undifferentiated Cells
Marker expression: SSEA-4(+)POU5F1 (OCT-4)(+)SSEA-1(-)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Not done
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: -
Genotyping
Karyotyping
Cell line karyotype: No autosomal or sex chromosome aneuploidies detected
Karyotyping method: Karyolite BoBsLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Matrigel®/Geltrex®
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: Essential E8®Legal InformationE8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation
EBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.Параметрыbiological source human dermis (fibroblast) reprogramming method Sendai virus description age (65-69) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) depression (unipolar) shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
EDi015-B 66540461-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of EdinburghCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown
Other:From the same donor: EDi015-A, EDi015-C
Characterization
Analysis of Undifferentiated Cells
Marker expression: SSEA-1(-)POU5F1 (OCT-4)(+)SSEA-4(+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Not done
Hepatitis C: Negative
Mycoplasma: -
Genotyping
Karyotyping
Cell line karyotype: No autosomal or sex chromosome aneuploidies detected
Karyotyping method: Karyolite BoBsLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Matrigel®/Geltrex®
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: Essential E8®Legal InformationE8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation
EBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.Параметрыbiological source human dermis (fibroblast) reprogramming method Sendai virus description age (65-69) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) depression (unipolar) shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
EDi015-C 66540462-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of EdinburghCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Is the reprogramming vector detectable: Unknown
Other:From the same donor: EDi015-A, EDi015-B
Characterization
Analysis of Undifferentiated Cells
Marker expression: SSEA-1(-)SSEA-4 (+)POU5F1 (OCT-4) (+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Not done
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: -
Genotyping
Karyotyping
Cell line karyotype: No autosomal or sex chromosome aneuploidies detectedLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Matrigel®/Geltrex®
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: Essential E8®Legal InformationE8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation
EBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.Параметрыbiological source human dermis (fibroblast) reprogramming method Sendai virus description age (75-79) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) depression (unipolar) shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
EDi016-A 66540463-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of EdinburghCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Is the reprogramming vector detectable: Unknown
Other:From the same donor: EDi016-C
Characterization
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Not done
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: -
Genotyping
Karyotyping
Passage number: 19
Cell line karyotype: No autosomal or sex chromosome aneuploidies were detected
Karyotyping method: BACs-on-BeadsLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Vitronectin
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: Essential E8®Legal InformationE8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation
EBiSC is a trademark of Fraunhofer-GesellschaftПараметрыbiological source human dermis (fibroblast) reprogramming method Sendai virus description age (N/A) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) none shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
EDi016-C 66540465-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of EdinburghCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown
Other:From the same donor: EDi016-A
Characterization
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Not done
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: -
Genotyping
Karyotyping
Passage number: 16
Cell line karyotype: No autosomal or sex chromosome aneuploidies were detected
Karyotyping method: BACs-on-BeadsLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Vitronectin
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: Essential E8®Legal InformationE8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation
EBiSC is a trademark of Fraunhofer-GesellschaftПараметрыbiological source human dermis (fibroblast) Quality Level 100, reprogramming method Sendai virus description age (N/A) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) none shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
EDi017-A 66540466-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
University of EdinburghCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown
Other: From the same donor: EDi017-B, EDi017-C
Characterization
Analysis of Undifferentiated Cells
Marker expression: TRA 1-60(+)POU5F1 (OCT-4)(+)SSEA-1(-)SSEA-4 (+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Not done
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: -
Genotyping
Karyotyping
Passage number: 23
Cell line karyotype: No autosomal or sex chromosome aneuplodies were detected
Karyotyping method: BACs-on-BeadsLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Matrigel®/Geltrex®
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: Essential E8®Legal InformationE8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation
EBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.Параметрыbiological source human dermis (fibroblast) reprogramming method Sendai virus description age (N/A) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) none shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
HUBi001-A 66540042-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
The Hubrecht InstituteCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Passage number reprogrammed: 25
Reprogramming method
Vector type: Integrating
Vector: Virus
Virus type: Lentivirus
Gene list:
KLF4
MYC
POU5F1
SOX2
Have the reprogramming vectors been silenced: Yes
Merthods used: rtpcr
Characterization
Analysis of Undifferentiated Cells
Marker expression: SSEA-1 (-)TRA 1-60 (+)SSEA-4 (+)
Differentiation potency
Ectoderm: BTUBULIN (-)FOXG1(-)HES5(+)NEUROD1(+)PAX6(-)Sox1(+)
Endoderm: CXCR4 (+)FOXA2(-)GATA6(+)GSC (-)SOX17(+)
Mesoderm: BMP4(+)DECORIN (+)GATA4(-)HAND1(+)PDGFRA(+)PECAM1(+)PITX1(-)VIMENTIN(+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: 37
Cell line karyotype: 46,XY
Karyotyping method: G-Banding
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 20%
Temperature: 37°CLegal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) reprogramming method lentivirus description age (N/A) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) none shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
LUBi001-B 66540258-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
H. Lundbeck A/SCell Line DescriptionDerivation
Primary cell type: Fibroblast
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown
Characterization
Analysis of Undifferentiated Cells
Marker expression: SSEA-4(+)SSEA-1(-)TRA 1-60(+)
Differentiation potency
Ectoderm: In vitro spontaneous differentiation
Marker Expressed:HES5(+)NeuroD1(+)Pax6(+)
Endoderm: In vitro spontaneous differentiation
Marker Expressed:CXCR4(+)GATA6(+)Sox17 (+)
Mesoderm: In vitro spontaneous differentiation
Marker Expressed: DCN(+)MIXL1(+)NCAM(+)PECAM (+)Vimentin(+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: 22
Cell line karyotype: TRISOMY 12 indicated in this sample
Karyotyping method: Karyolite BoBs
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: Essential E8®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 21%
Temperature: 37°CLegal InformationE8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation
EBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.Параметрыbiological source human dermis (fibroblast) reprogramming method Sendai virus description age (65-69) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) frontotemporal dementia shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
LUBi001-C 66540259-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
H. Lundbeck A/SCell Line DescriptionDerivation
Primary cell type: Fibroblast
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown
Characterization
Analysis of Undifferentiated Cells
Marker expression: SSEA-1(-)TRA 1-60(+)G77 SSEA-4 (+)
Differentiation potency
Ectoderm: In vitro spontaneous differentiation
Endoderm: In vitro spontaneous differentiation
Mesoderm: In vitro spontaneous differentiation
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: 18
Cell line karyotype: 46,XY
Karyotyping method: G-Banding
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA)
- Cell Line Information Pack (CLIP)
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: Essential E8®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 21%
Temperature: 37°CLegal InformationE8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation
EBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.ПараметрыQuality Level 100 biological source human dermis (fibroblast) reprogramming method Sendai virus description age (65-69) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) frontotemporal dementia shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point(F) Not applicable Flash Point(C) Not applicable Узнать цену -
PFIZi013-A 66540322-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Pfizer Limited - PfizerCell Line DescriptionDerivation
Primary cell type: Fibroblast
Passage number reprogrammed: 4
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown
Characterization
Marker expression: POU5F1 (OCT-4)(+)SSEA-1(-)SSEA-4 (+)TRA 1-60(+)
Differentiation potency:Ectoderm: HES5(+)NeuroD1(+)PAX6(+),EndodermGATA6 (+)SOX17 (+),Mesoderm: DCN(+)MIXL1(+)VIM (+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: 26
Cell line karyotype: No autosomal or sex chromosome aneuplodies were detected
Karyotyping method: BoBs
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA)
- Cell Line Information Pack (CLIP)
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: Essential E8®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 21%
Temperature: 37°CLegal InformationE8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation
EBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.ПараметрыQuality Level 100 biological source human dermis (fibroblast) reprogramming method Sendai virus description age (N/A) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) amyotrophic lateral sclerosis (ALS) shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point(F) Not applicable Flash Point(C) Not applicable Узнать цену -
RBi001-A 66540025-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
R BiomedicalCell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Primary cell developmental stage: Adult
Reprogramming method
Vector type: Non-integrating
Vector: Episomal
Is the reprogramming vector detectable: Unknown
Characterization
Analysis of Undifferentiated Cells
Marker expression: TRA 1-60(+)SSEA-4(+) POU5F1 (OCT-4)(+)
Differentiation potency
Ectoderm: In vitro spontaneous differentiation
Marker Expressed:HES5(+)NEUROD1 (+)PAX6(+)
Endoderm: In vitro spontaneous differentiation
Marker Expressed:CXCR4 (+)GATA6 (-)SOX17 (-)
Mesoderm: In vitro spontaneous differentiation
Marker Expressed:DCN(+)MIXL1(-)NCAM(+)VIMENTIN(+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: Essential E8®
Passage method: EDTA
Matrix: Vitronectin
CO2 concentration: 5%
O2 concentration: 21%
Temperature: 37°CLegal InformationE8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation
EBiSC is a trademark of Fraunhofer-GesellschaftПараметрыbiological source human dermis (fibroblast) Quality Level 100, reprogramming method episomal description age (45-49) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) none shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
RCi001-A 66540016-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Pfizer Limited - PfizerCell Line DescriptionDerivation
Primary cell type: Erythroblast
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown
Other:From the same donor: RCi001-B
Characterization
Analysis of Undifferentiated Cells
Marker expression: POU5F1 (OCT-4)(+)SSEA-4(+)SSEA-1(+) TRA 1-60(+)
Differentiation potency
Ectoderm: In vitro spontaneous differentiation
Endoderm: In vitro spontaneous differentiation
Mesoderm: In vitro spontaneous differentiation
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Not done
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: 11
Cell line karyotype: Normal
Karyotyping method: G-Banding
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 21%
Temperature: 37°CLegal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human erythroblast Quality Level 100, reprogramming method Sendai virus description age (N/A) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) erythromelalgia shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
RCi001-B 66540048-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Pfizer Limited - PfizerCell Line DescriptionDerivation
Primary cell type: Erythroblast
Primary cell developmental stage: Adult
Passage number reprogrammed: NA
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Is the reprogramming vector detectable: Unknown
Other:From the same donor: RCi001-A
Characterization
Analysis of Undifferentiated Cells
Marker expression: TRA 1-60(+)SSEA-1(-)SSEA-4(+)POU5F1 (OCT-4)(+)
Differentiation potency
Ectoderm: In vitro spontaneous differentiation
Endoderm: In vitro spontaneous differentiation
Mesoderm: In vitro spontaneous differentiation
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: 15
Cell line karyotype: 46,XY[14]/47,XY,+12[5]
Karyotyping method: G-Banding
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 21%
Temperature: 37°CLegal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human erythroblast Quality Level 100, reprogramming method Sendai virus description age (N/A) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) erythromelalgia shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
RCi002-A 66540049-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Pfizer Limited - PfizerCell Line DescriptionDerivation
Primary cell type: Erythroblast
Primary cell developmental stage: Adult
Passage number reprogrammed: NA
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown
Other:From the same donor: RCi002-B
Characterization
Analysis of Undifferentiated Cells
Marker expression: SSEA-4(+)SSEA-1(-)POU5F1 (OCT-4)(+) TRA 1-60(+)
Differentiation potency
Ectoderm: In vitro spontaneous differentiation
Endoderm: In vitro spontaneous differentiation
Mesoderm: In vitro spontaneous differentiation
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: P12
Cell line karyotype: 46XX
Karyotyping method: G-Banding
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 21%
Temperature: 37°CLegal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human erythroblast reprogramming method Sendai virus description age (N/A) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) erythromelalgia shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
RCi002-B 66540050-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Pfizer Limited - PfizerCell Line DescriptionDerivation
Primary cell type: Erythroblast
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown
Other:From the same donor: RCi002-A
Characterization
Analysis of Undifferentiated Cells
Marker expression: SSEA-4(+)SSEA-1(-)POU5F1 (OCT-4)(+)TRA 1-60(+)
Differentiation potency
Ectoderm: In vitro spontaneous differentiation
Endoderm: In vitro spontaneous differentiation
Mesoderm: In vitro spontaneous differentiation
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: P15
Cell line karyotype: 18 out of 20 cells normal, one cell 45,X,add(X)(q22),-11, one cell 45,XX,-22
Karyotyping method: G-Banding
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 21%
Temperature: 37°CLegal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human erythroblast reprogramming method Sendai virus description age (N/A) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) erythromelalgia shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
RCi003-A 66540051-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Pfizer Limited - PfizerCell Line DescriptionDerivation
Primary cell type: Erythroblast
Passage number reprogrammed: NA
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown
Other:From the same donor: RCi003-B
Characterization
Analysis of Undifferentiated Cells
Marker expression: TRA 1-60(+)POU5F1 (OCT-4)(+)SSEA-1(-)SSEA-4(+)
Differentiation potency
Ectoderm: In vitro spontaneous differentiation
Endoderm: In vitro spontaneous differentiation
Mesoderm: In vitro spontaneous differentiation
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: P15
Cell line karyotype: 14 out of 20 cells showed loss of chromosome 20 short arm and gain of long arm
Karyotyping method: G-Banding
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 21%
Temperature: 37°CLegal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human erythroblast reprogramming method Sendai virus description age (N/A) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) pain agnosia shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
RCi003-B 66540052-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Pfizer Limited - PfizerCell Line DescriptionDerivation
Primary cell type: Erythroblast
Primary cell developmental stage: Adult
Passage number reprogrammed: NA
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown
Other:From the same donor: RCi003-A
Characterization
Analysis of Undifferentiated Cells
Marker expression: SSEA-1(-)SSEA-4(+)TRA 1-60(+)POU5F1 (OCT-4)(+)
Differentiation potency
Ectoderm: In vitro spontaneous differentiation
Endoderm: In vitro spontaneous differentiation
Mesoderm: In vitro spontaneous differentiation
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: P16
Cell line karyotype: 18 cells out of 20 normal, 1 cell 47,XXX, one cell 45,XX,-17
Karyotyping method: G-Banding
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 21%
Temperature: 37°CLegal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human erythroblast Quality Level 100, reprogramming method Sendai virus description age (N/A) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) pain agnosia shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
RCi004-A 66540024-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Roslin Cells Ltd.Cell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Primary cell developmental stage: Adult
Passage number reprogrammed: P4
Reprogramming method
Vector type: Non-integrating
Vector: Episomal
Gene list:
KLF4
Lin28
MYC
POU5F1
shP53
SOX2
Is the reprogramming vector detectable: Unknown
Other:From the same donor: RCi004-B
Characterization
Analysis of Undifferentiated Cells
Marker expression: POU5F1 (OCT-4)(+)SSEA-1(-)TRA 1-60(+)SSEA-4 (+)
Differentiation potency
Ectoderm: In vitro spontaneous differentiation
Marker Expressed:B-TUBULIN(-)FOXG1(+)HES5(+)NEUROD1(+)PAX6(+)Sox1(+)
Endoderm: In vitro spontaneous differentiation
Marker Expressed:CXCR4 (+)FOXA2(+)GSC(+)PITX1(+)SOX17(+)
Mesoderm:In vitro spontaneous differentiation
Marker Expressed: BMP4 (+)DCN(+)GATA4(+)HAND1(+)PDGF(+)PECAM(+)VIMENTIN(+)
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: P16
Cell line karyotype: 46,XX,i(20)(q10)
Karyotyping method: G-Banding
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 21%
Temperature: 37°CLegal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) Quality Level 100, reprogramming method episomal description age (N/A) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) Huntingtons disease shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
RCi004-B 66540014-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Roslin Cells Ltd.Cell Line DescriptionDerivation
Primary cell type: Fibroblast of dermis
Primary cell developmental stage: Adult
Location of primary tissue procurement: Edinburgh
Passage number reprogrammed: 4
Reprogramming method
Vector type: Non-integrating
Vector: Episomal
Gene list:
KLF4
Lin28
MYC
POU5F1
shP53
SOX2
Is the reprogramming vector detectable: Unknown
Characterization
Analysis of Undifferentiated Cells
Marker expression: TRA 1-60(+)SSEA-1(-)POU5F1 (OCT-4)(+)SSEA-4(+)
Differentiation potency
Ectoderm: In vitro spontaneous differentiation
Marker Expressed:B-TUBULIN(-)FOXG1(-)HES5(+)NEUROD1(+)PAX6(+)Sox1(-)
Endoderm: In vitro spontaneous differentiation
Marker Expressed:CXCR4(+)FOXA2(+)GATA6 (+)GSC(-)PITX1(+)SOX17(+)
Mesoderm: In vitro spontaneous differentiation
Marker Expressed:BMP4(+)DCN(+)GATA4(+)HAND1(+)PDGF(-)PECAM (-)VIMENTIN(+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: P15
Cell line karyotype: 14 out of 20 cells normal, 4 cells loss of chromosome 20 short arm and gain of long arm. 2 cells 46,XX,add(16)(p13.1)
Karyotyping method: G-Banding
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 21%
Temperature: 37°CLegal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.Параметрыbiological source human dermis (fibroblast) reprogramming method episomal description age (N/A) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) Huntingtons disease shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
RCi005-A 66540044-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Roslin Cells Ltd.Cell Line DescriptionDerivation
Primary cell type: Fibroblast
Reprogramming method
Vector type: Non-integrating
Vector: Episomal
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown
Characterization
Analysis of Undifferentiated Cells
Marker expression: SSEA-1(-)SSEA-4(+)TRA 1-60(+)
Differentiation potency
Ectoderm: In vitro spontaneous differentiation
Endoderm: In vitro spontaneous differentiation
Mesoderm: In vitro spontaneous differentiation
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: -
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: 27
Cell line karyotype: No autosomal or sex chromosome aneuplodies were detected
Karyotyping method: Karyolite BoBs
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Matrigel®/Geltrex®
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 21%
CO2 concentration: 5%
Culture medium: Essential E8®Legal InformationE8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation
EBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.Параметрыbiological source human dermis (fibroblast) Quality Level 100, reprogramming method episomal description age (45-49) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) facioscapulohumeral muscular dystrophy shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
RCi006-A 66540107-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Roslin Cells Ltd.Cell Line DescriptionDerivation
Primary cell type: Fibroblast
Passage number reprogrammed: 2
Reprogramming method
Vector type: Non-integrating
Vector: Episomal
Gene list:
KLF4
MYC
OCT4
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown
Characterization
Analysis of Undifferentiated Cells
Marker expression: SSEA-4(+)POU5F1 (OCT-4)(+)SSEA1(-)
Differentiation potency
Ectoderm: In vitro spontaneous differentiation
Marker Expressed:HES5(+)NEUROD1(+)PAX6(+)
Endoderm: In vitro spontaneous differentiation
Marker Expressed:CXCR4(+)GATA6(+)SOX17 (+)
Mesoderm: In vitro spontaneous differentiation
Marker Expressed:DCN(+)VIMENTIN(+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: 11
Cell line karyotype: 46,XY
Karyotyping method: G-Banding
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: Essential E8®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 21%
Temperature: 37°CLegal InformationE8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation
EBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.Параметрыbiological source human dermis (fibroblast) Quality Level 100, reprogramming method episomal description age (60-64) Торговая марка EBiSC™ gender male growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) facioscapulohumeral muscular dystrophy shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
RCi007-A 66540045-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Roslin CellsCell Line DescriptionDerivation
Primary cell type: Fibroblast
Passage number reprogrammed: 4
Reprogramming method
Vector type: Non-integrating
Vector: Episomal
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
SSEA-1 No
SSEA-4 Yes
TRA 1-60 Yes
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Genotyping
Karyotyping
Passage number: 17
Cell line karyotype: 46,XX
Karyotyping method: G-BandingLinkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineSurface coating: Matrigel®/Geltrex®
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 21%
CO2 concentration: 5%
Culture medium: Essential E8®Legal InformationE8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation
EBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.Параметрыbiological source human dermis (fibroblast) reprogramming method episomal description age (55-59) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) facioscapulohumeral muscular dystrophy shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
RCi007-C 66540163-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Roslin Cells Ltd.Cell Line DescriptionDerivation
Primary cell type: Fibroblast
Passage number reprogrammed: 4
Reprogramming method
Vector type: Non-integrating
Vector: Episomal
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown
Characterization
Analysis of Undifferentiated Cells
Marker expression: SSEA-4(+)SSEA-1(-)TRA 1-60(+)
Differentiation potency
Ectoderm: In vitro spontaneous differentiation
Endoderm: In vitro spontaneous differentiation
Mesoderm: In vitro spontaneous differentiation
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: -
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: 15
Cell line karyotype: 46,XX
Karyotyping method: G-Banding
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: Essential E8®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 21%
Temperature: 37°CLegal InformationE8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation
EBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.Параметрыbiological source human dermis (fibroblast) Quality Level 100, reprogramming method episomal description age (55-59) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) facioscapulohumeral muscular dystrophy shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
RCi009-A 66540260-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Roslin Cells Ltd.Cell Line DescriptionDerivation
Primary cell type: Fibroblast
Reprogramming method
Vector type: Non-integrating
Vector: Episomal
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown
Characterization
Analysis of Undifferentiated Cells
Marker expression: TRA 1-60(+)SSEA-1(-)POU5F1 (OCT-4)(+)SSEA-4(+)
Differentiation potency
Ectoderm: In vitro spontaneous differentiation
Marker Expressed:HES5(+)NEUROD1(+)PAX6(+)
Endoderm: In vitro spontaneous differentiation
Marker Expressed:CXCR4(+)GATA6(+)SOX17(+)
Mesoderm: In vitro spontaneous differentiation
Marker Expressed:DCN(+)MLX1(+)VIMENTIN(+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: 15
Cell line karyotype: No autosomal or sex chromosome anueplodies detected
Karyotyping method: BoBs
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.Linkage- EBiSC Access Use Agreement (EAUA),
- Cell Line Information Pack (CLIP),
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: Essential E8®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 20%
Temperature: 37°CLegal InformationE8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation
EBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.Параметрыbiological source human dermis (fibroblast) reprogramming method episomal description age (50-54) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) facioscapulohumeral muscular dystrophy shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
SIGi001-A-1 66540356-1VL
General descriptionInduced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.Cell Line OriginDepositor
Sigma-AldrichCell Line DescriptionDerivation
Primary cell type: Epithelium
Reprogramming method
Vector type: Integrating
Vector: Virus
Virus type: Retrovirus
Gene list:
KLF4
MYC
POU5F1
SOX2
Have the reprogramming vectors been silenced: Unknown
Characterization
Analysis of Undifferentiated Cells
Marker expression: SSEA-1(-)SSEA-4 (+)POU5F1 (OCT-4) (+)TRA 1-60 (+)
Differentiation potency
Ectoderm: In vitro spontaneous differentiation
Marker Expressed:PAX6 (+)
Endoderm: In vitro spontaneous differentiation
Marker Expressed:GATA6 (+)
Mesoderm: In vitro spontaneous differentiation
Marker Expressed:AFP (+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: 37
Cell line karyotype: No autosomal or sex chromosome abnormalities detected
Karyotyping method: Karyolite BoBs
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.
Genetic Modification
Disease/phenotype related modifications
Disease: Corticobasal degeneration
There are three disease associations for the edited subclone: PSP (progressive supranuclear palsy) /CBD (Corticobasal degeneration) / FTDP-17 (Frontotemporal dementia and parkinsonism linked to chromosome 17) - like symptoms
Type of modification: Isogenic
Gene: MAPT
Chromosome location: 17q21.31
Zygosity: Homozygous
Target locus modification: Mutated
Description: P301S+IVS10+16 C>T
Disease: Progressive supranuclear palsy
There are three disease associations for the edited subclone: PSP (progressive supranuclear palsy) /CBD (Corticobasal degeneration) / FTDP-17 (Frontotemporal dementia and parkinsonism linked to chromosome 17) - like symptomsLinkage- EBiSC Access Use Agreement (EAUA)
- Cell Line Information Pack (CLIP)
Note: EAUA and CLIP must be completed before order fulfillmentSubculture RoutineMedium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 21%
Temperature: 37°CLegal InformationEBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.ПараметрыQuality Level 100 biological source human epithelium reprogramming method retrovirus description age (20-24) Торговая марка EBiSC™ gender female growth mode adherent (pluripotent) application(s) cell culture | stem cell: suitable relevant disease(s) none shipped in dry ice storage temp. 196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point(F) Not applicable Flash Point(C) Not applicable Узнать цену
Получите коммерческое предложение в течение 1 часа
Менеджер подготовит коммерческое предложение и позвонит, если понадобится уточнить детали вашего заказа
Анна Гловацкая
Менеджер по работе с клиентами
С 2010 года мы поставляем оборудование с заводов Европы. Берем на себя все — от подбора оборудования до внедрения на предприятии
Все сотрудники имеют высшее образование, закончили ведущие химические вузы страны, такие как РХТУ им Менделеева.
У большинства компаний срок ожидания составляет 10-12 недель.
Оборудование хранится на сухом отапливаемом складе, где поддерживается ровная температура.
Работаем с PonyExpress и Деловыми линиями. Вы также можете выбрать свою транспортную компанию или забрать товар со склада в Москве.
В случае любых неполадок за свой счет выполним ремонт в сервисном центре или на заводе-изготовителе. Или бесплатно заменим прибор на новый.
Производим пуско-наладку оборудования, валидацию, обучение сотрудников. Если нужно, привлекаем инженеров с заводов- изготовителей.
