Protease from Streptomyces sp.

CAS Number:	9036-06-0
EC Number:	232-909-5
MDL number:	MFCD00132092
NACRES:	NA.54

Кат. номер

P0652-500UN

P0652-1KU

P0652-100UN

Pronase E can be used to degrade antheraea pernyi silk fibroin films.
This enzyme is useful for proteolysis of insoluble protein and for structure investigation of protein. Proteases from Streptomyces griseus and Streptomyces omiyaensis have been used in a study to identify residue 71 as a crucial residue for differences in topological specificities. Proteases have also been used in a study to investigate production of protease as a by-product of streptomycin production.
Protease is typically used in nucleic acid isolation procedures in incubations of 0.5-3.0 hours supplemented with 0.2% sodium dodecyl sulfate and 10 mM EDTA.
Completely inactivated by heating above 80 °C for 15-20 minutes.
A mixture of at least three proteolytic activities including an extracellular serine protease. In general, serine proteases display a wide range of substrate specificities, which are believed to be mediated by an active site composed of one Asp, one His, and a Ser residue in the molecule. This enzyme prefers to hydrolyze peptide bonds on the carboxyl side of glutamic or aspartic acid.
Packaging
500 units in glass bottle
Biochem/physiol Actions
Alkaline protease that is approximately twice as active at pH 11.0 than at the usual assay conditions for protease, pH 7.5 and 37 °C. By comparison, P5147 Type XIV protease is only approximately 25% as active at pH 11.0, 30 °C.
Physical properties
Isoelectric point : 8.7
Inhibitors : Diisopropyl fluorophosphate, EDTA
Optimum pH : >=12
Optimum temperature : 60oC
pH Stability : pH 5.0 - 11.5 (25oC,24hr)
Thermal stability : below 50oC (pH 8.3, 15min)
Unit Definition
One unit will hydrolyze casein to produce peptide equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 11.0 at 30 °C.
Physical form
This enzyme is more active at a higher pH range than the known alkaline protease, showing the proteolytic activity even in 0.2N NaOH solution. This enzyme is useful for proteolysis of insoluble protein and for structure investigation of protein.
Preparation Note
Collected from culture broth of S.Sp

type	Type XXI
Quality Level	200,
form	powder
specific activity	15 units/mg solid
mol wt	~50 kDa
storage temp.	20°C

pictograms	GHS07,GHS08
signalword	Danger
hcodes	H315 - H319 - H334 - H335
pcodes	P302 + P352 - P305 + P351 + P338
Target Organs	Respiratory system
Personal Protective Equipment	Eyeshields,, Gloves,, type N95 (US),
RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	Not applicable
Flash Point C	Not applicable